Randy Holmes-Farley said:
Here's a recipe for a standard (for refractometers only):
Title photo by R2R member Rskillz By Randy Holmes-Farley Salinity is one of the most important parameters measured in reef aquaria. It controls not only the salt balance between an organism and its surrounding environment, but also the levels of a host of ions Read more here...
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Do-it-yourself Refractometer Standards
In a
previous article I described how to make a do-it-yourself refractometer standard matching 35 ppt seawater, and I will just summarize that recipe here.
To provide a standard for refractometers requires a solution whose refractive index is similar to normal seawater. Seawater with a salinity of 35 ppt has a refractive index of 1.3394. Likewise, the refractive index of different sodium chloride solutions can be found in the scientific literature. My CRC Handbook of Chemistry and Physics (57th Edition, Page D-252) has such a table. That table has entries for 3.6 and 3.7 weight percent solutions of sodium chloride that span the value for normal seawater. Interpolating between these data points suggests that a solution of 3.65 weight percent sodium chloride has the same refractive index as 35 ppt seawater, and therefore can be used as an appropriate standard (Table 5).
This 3.65 weight percent sodium chloride solution can be made by dissolving 3.65 grams of sodium chloride in 96.35 grams (mL) of purified freshwater. This recipe can be scaled to any appropriate size if suitable instruments are available (36.5 grams in 963.5 grams (mL) of water, 0.365 grams in 9.635 g (mL) of water, etc.).
This concentration roughly corresponds to ¼ cup (73.1 g) of Morton’s Iodized Salt dissolved into two liters (2000 g) of water (giving very slightly more than 2 L of total volume).