Adding fertilizer half way through Phytoplankton culture

[LardLad]

Does anyone add fertilizer halfway through their phytoplankton culture?

I use 7-10 day cycles, Tetraselmis, F2 @ 1ml/liter, 16/8 photoperiod (10k LED) @ 70F.

 

[NeonRabbit221B]

I have always had them crash if I redose fert. Bumping for knowledge as I am growing nano to start

 

[LardLad]

I have always had them crash if I redose fert. Bumping for knowledge as I am growing nano to start

Thanks. Are you doing similar params to me? F2 1ml/liter?

 

[NeonRabbit221B]

I think I was doing 10 drops per L so that should be half that... Don't trust me on that but I can check when I get home.

 

[Subsea]

I use modified F2 from Mercer of Montana at 1ml/L, except I tripled the dosage and harvest 4L every other day.

I also quadrupled light source and run 24/7. Extra heat required a fan to cool culture down to 79 degrees.

 

[Mr. Mojo Rising]

i never add fert halfway through. I use guillards, the instruction is .5 ml per litre, but i always put about double that, so about 2 ml in my 2 littre bottle. In 5-6 days my stuff is dark green and put it in the fridge.

 

[fryman]

Why does everyone increase fert doseage? The recommended dose is such for a reason, studies were done to prove this is the optimal dose. If you have to add more, it's probably because you are growing something else besides the phyto.

I split my culture after 6-7 days and do not add fert. I have added fert after 7 days to prevent a crash if I'm busy or just being lazy. The culture gets a little darker in the 2nd week but growth slows alot and it's probably not as good. Phyto is most nutricious during the exponential growth phase.

 

[NeonRabbit221B]

Bumping this for knowledge. Starter culture always does great and even after I split it I usually got a good second yield but that third split has killed my last two disks. Any tips on why I crash after 2 splits? Typically crashes about 4 days after the 2nd split.

 

[fryman]

Bumping this for knowledge. Starter culture always does great and even after I split it I usually got a good second yield but that third split has killed my last two disks. Any tips on why I crash after 2 splits? Typically crashes about 4 days after the 2nd split.

Maybe contamination. How are you cleanimg between splits?

I usually bleach everything, at most go 1 split between sanitizations or bacterial contamination can get really bad.

 

[NeonRabbit221B]

Maybe contamination. How are you cleanimg between splits?

I usually bleach everything, at most go 1 split between sanitizations or bacterial contamination can get really bad.

Bleach usually for bottles and rigid tubing but this most recent split I didn't boil the water to sterilize it. Instead I cleaned out the bucket and wiped it with bleach and waited 24 hours. I am wondering if I still had bleach in the bottles or if its something else. Restarted the cultures tonight with what I harvested Friday. Still have 1 culture that was going strong so I left that one and just restarted the other three.

Scratching my head on contamination issues as if the other three were killed off, why not the first?