I think for this particular kit, .25 is zero
its not always a bad kit, threads show good kit procedure too. we sometimes find people using freshwater reference cards on saltwater reads, nonshaking of reagents correctly, and fill line inconsistencies and the tests are sensitive to those variables. certain tank additives like Prime also skew results. in the end, false readings are occurring no matter how we want to attribute them.
*API is better suited for identifying large spikes, like a true dead fish or turnover from a bad sandbed incident.
any sustained .25's test readings coming from a system that already nitrifies at least .5-1.0 ppm of ammonia everyday off tank bioload is suspect, not the microbiology. it won’t really be stopping at .25, the filter system is getting all the ammonia
Its hard to know when to doubt a test reading with all the confounds. Your results are not drastically different than other search results using the same numbers