100%. They could have relocated into my cheato. When the bubbles were going through my main tank, it seemed like they died off. Once the bubbles stopped, there was only a handful left and only a few of them were large. I am starting on the left side of the sump and will work my way to the right. Even if they are released, eventually there will be nowhere to go except into the main display tank. My plan is eventually to put the airstones in my return pump and fill my display tank with a bunch of bubbles for a week or so.