22gallons of water volume roughly in display so sump and all I’d say close to 25 yesI have never used the product but the instructions say
1ml/6.5 gallons contributes 0.176 meq/L convert that to 0.495 dKH
going to assume your 32 gallon biocube has 25 gallons of water so
1ml/25 gallons contributes 0.129 dKH you added 4ml so multiply that by 4 give you 0.5148 dKH
You could also use the calculator that Randy posted



