Randy the goal is to aid in reducing no3 and po4 by from time to time adding different bacteria strains to the system. From what I have read, and I would appreciate any articles you favor in this area, Nitrobactor and Nitrosommas need oxygen and are better denitrifiers then the bacillus gang which seem to be no oxygen types. I know how you feel about multiple strains of bacteria in a system. That is eventually the faster growing will dominate or outcompete the slower grower. The Nitro brothers being the slower grower, but possibly the more valuable one in denitrification. Problem I see is the Nitro brothers die in a bottle on the LFS shelves. They dont go to seed and regrow as I think the bacillus family do. Bacillus has a much longer shelf life. I have never used bacteria other than to start a new system; however some systems maybe bacteria deficient for many reasons. Adding once or twice a year maybe beneficial to establish new strands? Thoughts and reading list? Thanks