75-90 minutes in the bath (bucket) then into the DT. From Humblefish's treatment advisory.
Acriflavine: This is an antiseptic that is very useful for rendering “first aid” if a fish has a bacterial infection or to treat an open wound. It can also be used as part of a “three step program” for treating Marine Velvet Disease: 5 minute freshwater dip, 75-90 minute acriflavine bath, and then prolonged exposure to copper or Chloroquine phosphate in a Quarantine Tank (QT).
There is also some anecdotal evidence that a 75-90 minute acriflavine bath, followed by transfer into a sterile Quarantine Tank can clear Brooklynella aka clownfish disease. However, until more research is done it is recommended to follow up the acriflavine bath by treating with metronidazole in the Quarantine Tank.
How To Treat – At present time there are two products on the market which contain acriflavine (dosage information below). Use either in a 75-90 minute bath, temperature controlling the water and providing plenty of aeration by using an air pump & air-stone. Ensure salinity, pH and temperature of bath & QT water match perfectly.
Acriflavine-MS: Add 1 teaspoon per 5 gallons of water.
Ruby Reef Rally: Add 1 ounce per 10 gallons of water. This breaks down to roughly 2/3 teaspoon of Rally per gallon if using less than 10 gallons.
Pros – Effective “pre-treatment” before fish is placed in QT with copper, antibiotics, etc.; milder/safer alternative to using formalin.
Cons/Side Effects – Turns the water an orange-brownish color, so probably not desirable for Quarantine or Display Tank use. Full range of positive effects and possible side effects are not completely understood at this time.