here's our roadmap to not lose them, curious to know what differed. we're out to 23 pages lost one goby only. can't be tied to the rinse imo
If you are reading this thread to cure a tank invasion from a link I sent you, we do not need to identify your type of invasion here we do not need you to test anything at anytime regarding nitrate, phosphate etc Above all, we do not need to see a microscope slide picture of your invasion at...
www.reef2reef.com
did you rinse your bed in tap water before setting it back up? Nearly everyone on the planet would say no. Ironically, that's what makes our rinse thread never have losses. admit that's weird, rinse your sand in tap and your fish live, do it without tap water as a limited rinse using prepped water (to try and save bac, which they don't need our help) and the fish die because the rinsed sand could not pass a drop test. There's not enough made water to rinse an active bed with; it'll run out long before tap. Tap allows two hours rinsing if that's what it takes to pass a drop test (where the bed is disturbed on video, for proof we can see it was thorough rinsed)
did you have a video of the post rinsed sand, to show if it was clearly rinsed? If it was, then the fish loss isn't related to sand rinsing as 100% rinse = 100% no ammonia produced.
the bacteria in a sandbed don't matter, you can instantly remove them without harm in any reef and rocks take over, instantly. we think most losses associated with sandbed access come from trying to preserve the sandbed bacteria/under rinsing.
*if fish cannot handle being acclimated from holding containers back and forth that may have a role, but the trip home from LFS is usually worse so this is just a guess.