So far observations that I've made over the last 71 hours,
- initial dose made both tanks cloudy for ~30 minutes.
-24 hours in and bryopsis was showing signs of stress. Leafy structure was starting g to develop a slight curl.
-all livestock was still showing all normal activity, no signs of stress. Nothing else affected.
-Phosphates all and nitrates were still at same levels.
- around 46 hours in bryopsis was showing even more signs of weakening. The distinct fern leaf structure was fading and most of the bryopsis was becoming stringy like and laying down more rather than standing upright towards the light. Color was fading from green and turning pale.
- all fish still maintaining their appetite and all corals,fish and inverts still showing good signs of health.
- Chaeto still remains intact and no signs of stress. Bubble algae doesn't seem to be affected.
- phosphates and nitrates still remain relatively the same, maybe a slight uptick but not very noticeable comparing to the charts.
- I've noticed pods in bryopsis in the 29g biocube. Not sure if they were there previously but there is a significant amount of pods moving about throughout the dying bryopsis.
- 71 hours into treatment and bryopsis is beginning to thin out significantly more compared to day 1 of treatment.
- 3 days into treatment and fish are still eating and showing strong signs of health as far as eating and being active. Corals and inverts still doing well also. Chaeto and bubble algae still appear the same as they were prior to treatment.
In my 70g tank my 14 year old female clown got an abrasion behind her head. Not sure if she was injured from being aggressive with another fish or if she cut herself on a coral or rock. She's had abrasions and cuts from time to time but it's something that I'll have to keep an eye on during treatment.
-I will not check phosphates and nitrates over the next couple of days since their hasn't been much change.
- my 29 biocube doesn't have a skimmer and on my 70g the skimmer is off during treatment.i pulled all gfo and GAC from both tanks. I have a filter sock on the 70g drain in the sump and filter flows in chamber 2 media basket on the 29g.
- last water change was 1-22-17, 3 days prior to treatment on both tanks.
- other than bryopsis and possibly other die off, any change in phoshates and nitrates can be attributed to all of these routine maintenance and water filtration methods.
- like others have reported, I have noticed that in some spots in both tanks with lower or little lighting that the decline in bryopsis is much slower. I might increase led light input in both tanks for a couple of days to see if it helps. Still undecided though at the moment.
- I also did not account for rock work or other displacement pieces when calculating dosage. So in my case I might be a little over the calculated guideline dosage amounts that was laid out. In the 70g setup I might be slightly over but I'm pretty sure I dosed more than what was recommended in the guidelines in this thread.
As I've already observed, there hasn't been any declines in any of the organisms in both tanks.
-last thing to add so far is that I have continued to monitor and supplement the main water parameters.
Pretty sure I've missed something here and there but staying observant may help in the future and can improve upon what we know so far about treating bryopsis with fluconazole.
Hope this wasn't too much info.