Bob Goemans correctly uses the term 'anoxic' to describe a zone that contains an oxygen level of approximately 0.5 to 2.0 mg/litre and >>> directly due to this<<< destructive denitrification occurs ( NO3 to nitrogen gas).
The often & incorrectly term used 'anaerobic' represents a zone that contains less oxygen than the anoxic condition, and >>> directly due to this <<< the
ammonification form of denitrification occurs (NO3 is converted back to ammonia). Hydrogen sulphide will also form in an anaerobic zone, so creating such an environment must be avoided.
Substrate grain size & depth, along with flow rate through, are key considerations for success in this type of nutrient processing method especially if a thick substrate is being utilised. Removal of organic particulate matter from the water entering the substrate would be necessary. But over time channelling can occur leaving dead spots - anaerobic zones. If fine sediments eventually have high organic matter loading they tend to go anoxic/anaerobic at very shallow depths - between a few centimetres to a few millimetres.
This method is not set & forget. Organic matter build up in the substrate, compaction of the substrate & channelling of flow through the substrate can cause subtle changes that can lead to disaster over time.
Some interesting reading onthe subject I found a while back
The Plenum Method…… by Bob Goemans
http://www.saltcorner.com/Articles/Showarticle.php?articleID=117
Sandbeds - Part I by Bob Goemans and Sam Gamble
http://www.saltcorner.com/Articles/Showarticle.php?articleID=22
Sandbeds - Part II by Bob Goemans and Sam Gamble
http://www.saltcorner.com/Articles/Showarticle.php?articleID=23
Jaubert's Method, The 'Monaco System,' Defined And Refined
By Julian Sprung
http://www.advancedaquarist.com/2002/9/aafeature
ORP meter reading in deep sand bed
As mentioned above, this order of electron acceptors used to oxidize organic material is oxygen (O2), then nitrate (NO3-), then manganese (Mn++++), then iron (Fe++), then sulfate (SO4--). Researchers can plot the concentrations of these chemicals as a function of depth, and can also associate an
ORP with each transition, although some overlap of the chemistries takes place in each zone. The oxygen zone has an ORP of 0 to 600 mV, the nitrate zone is -150 to 550 mV, the manganese zone is -50 to 400 mV, the iron zone is -700 to -150 mV and the sulfate reduction zone is -850 to 0 mV. Consequently, if hydrogen sulfide is being formed from sulfate, the ORP is likely below 0 mv in that region.