When a nitrifying biofilm grows on crushed shell and or aragonite the biofilm activities will have little effect on the pH and total alkalinity.
The pH measured in the water column will be different from the pH measured within the microbial community of a biofilm and the acids produced locally may dissolve the carbon carbonate on which the film grows. Also at system pH of 8.2 .
Crushed shells (oyster shell) can be used in a marine aquarium system, in biofilters and bioreactors. Using crushed shell in stead of sand as a substrate for a growing biofilm in an aquarium, in a biofilter or reactor, will prevent alkalinity may be depleted by autotrophic nitrifying and de-nitrifying processes taking place in a biofilter and or the aquarium. Some calcium will be produced . Crushed shell is used in marine aquaria for many decades, aragonite was not always common available. Crushed shells are part of the so called " Bio", used in Europe since the sixties, to stabilise pH and alkalinity, as advised by F.de Graaf. ( ref: FDeGraaf1969 >
Frank De Graaf. Handboek voor het tropisch zeeaquarium. Tweede druk. A.J.G. Strengholt N.V. Amsterdam, 1969. Graaf, Frank de. Marine Aquarium Guide. Harrison, N.J.: Pet Library, 1973. ) In the beginning crushed shells where used in the second compartment after the sand compartment. As nitrification mainly took place in the sand this did not work very good, mainly because the shell grit became bio-fouled. (SSpotte1970 >
Spotte, Stephen. Fish and invertebrate culture; water management in closed systems. New York: Wiley-Interscience, 1970. ) Replacing the sand in the first compartment of the "bio" by crushed shell solved the "biofilter syndrome" in a marine aquarium.