I had better luck once I used bleach/ thiosulfate to chlorinate and dechlorinate batches.
Looks neat but I would need the 200l barrel sized version of it to feed my larvae...
Ah got it. Sorry didn't know scale.
I'm thinking of switching to 30 or even 44 gallon Brute garbage cans for my Parvocalanus cultures, not just for volume but also for the ability to heat them better (the garage gets cold in the winter). I tried rectangular storage bins but the flow in those isn't ideal for harvesting nauplii.
There is a big box under my Christmas tree from poseidon not sure if the wife knows that I know about it or not. She thinks she is sneaky but i grab the packages every day....
If I got this project going I may have to look into that for storage purposes. I know that Iso doesn't do well in the refrigerator and prefers to be stored at room temp.
The only things that touched one live culture on the way to the next flask of sterilized media ready to grow were a 10 micron filter and a funnel. And those both got a rinse in peroxide then distilled water first. Coulda used isopropyl instead, I guess.
This may be the last time that I order from Algae Research Supply. They sent me a starter culture that was brown as Iso is, but I don't have a microscope to confirm it. now all of my cultures are turning green rather than brown like Iso should be. I think that they sent me a culture that was contaminated with Nano. i'm not culturing any other algaes so it has to be from the starter. I would love to get a pure starter so that I can culture this algae and grow Parvocalinas copepods. i'm feeding paste to my parvo now and it seems to be sustaining them but it's not making them boom in numbers that I am accustomed to like Apocyclops or Tigger pods.
