You may have an acrylic fabricator near you, you can possibly get an off-cut for cheap.
There are a bunch of online acrylic vendors.
The big box stores carry acrylic as well, although it isn't the best quality stuff. But you aren't building the sump or aquarium out of it, so it doesn't really matter.
I'd check for a local fabricator or vendor first.
You can ghetto fab it with a dremmel.
Wood saws work on acrylic. Hole saw, skill, or jig saw. Who says the hole needs to be circular? You can make your own socks and rings to hold them in place.
If you have a drill press, you can go scray fab and use one of those adjustable circle cutters on a low speed. They work well (and are cheap), but if you don't have them set up correctly and run them on high speed, they are quite dangerous. You also need a drill press that has a decent mass to it or is bolted down. Nothing like having an unballanced mass spinning at a high rate of speed. (Not recommended for the uninitiated or for use with hand held drills)
You could also contact one of the acrylic fabricators on here and see if they will make it for you. You supply the dimensions. If you need to ask the question, you probably need to buy the tools. It may be cheaper in the long run to have someone make it. Are you realistically going to use the tools need to make it again?
I haven't (intentionally) made curved cuts in glass, I'm not sure you would be able to cut a 4" circle out of glass with a normal glass cutter. I've seen it done with a circle scriber in the movies.....
I know the process for notching a glass panel, but I have never done it myself and it is a very different process from removing a circle from the center of a panel. Maybe a glass shop could do it for you.