Just my two cents bacteria in a bottle is nothing but waste of money.
Which ones have you used?
I have used the ProdiBio (startup) system, and was good to add fish after 72 hours. I also used weekly supplements for 6-8 week afterwards. This was many years ago when it was a very new (and expensive) way to do things.
I also know someone who used the Aquavitro Seed product succesfully. It was safely tested to add fish inside of one week.
I do understand this concept is controversial, which it should be with all 'new' ways of doing things. However, it is important that OP has all the information available to him/her so he/she can make the decision he/she is most comfortable with. That said, if I were to use these productions again, I would 'over dose' them just to be safe. Also, it is important to not do a water change right away (give it a couple-few weeks); since you would be dosing into the water column, it would take some time for the bacteria to establish onto the rocks. It is also important to 'ghost feed' throughout the dosing period, this way you are introducing a food/ammonia/nitrogen-cycle source for the bacteria to feed on. The science is fairly sound on dosing bacteria, but differentiating snake oil from effective products is likely the challenge here...
Lastly, I am a huge proponent of the 'instant' cycle that all live rock provides... In a tank this small, $50 in live rock would be more than enough to skip over all this fuss...
