Thank you for the kind words. I have had a series of events leading up to now. My fish were in a 20 gallon waiting for my new tank to get set up. My old 90 gallon had Dino's, identified by microscope, so bad that I lost my coral and all the rest of my inverts and decided to tear it down. I had been wanting a better tank and after doing some research decided on the Red Sea Reefer 450. Setting it up has not gone as quickly as I hoped and my guys were downstairs waiting. I have been having trouble with nitrates, over 25, and phosphates, between 2-5. I knew it must be over feeding so along with less food decided to try nopox. I added 1ml to the 22gallon last evening after a water change. At the same time my 2 spot bristletooth tang had what were small white parasites clinging to him so I had added 1 tsp of Prazi to the tank Sunday and a second dose last night because he still had quite a few on him. These parasites had been an on again off again issue for him for a long time. None of the others showed any on them. Could he have died during the night and the water fouled killing everyone?