If you clean them out fully no harm comes to the nano, it just looks cleaner, the cycle cannot be undone. any form of new benthic growth signifies your cycle is done; what non seneye ammonia tests say won’t factor, that’s a biological confirmation.
you are now into the options of the uglies or just using the accessibility a nano affords to just clean it all. Do a 100% water change if you want, rinse out the sand and rocks then you’ll have no diatoms. We deep clean tanks all the time as prep to moving homes. It’s no different for you, though your tank is staying there.
you can also let that + new invaders after it take over and do zero cleaning, many opt for that too. I wanted to introduce the notion of never allowing a nano to look bad from day one to day 2000, many have opted for that too and they had no invasions the whole time, they were simply cleaned out. Many people don’t want that, it’s too easy, it’s a cheat. They want gha to take over everything, we are told that’s part of the cycle. Can choose.