Nice write up! I just made a refrigerated food dispenser myself- it is definitely a worthwhile project. I'll have to do a write up, as the design is different, much cheaper, and the food last a long time- but that will have to wait until after Christmas. I will say that having taste tested thawed frozen mysis refrigerated at 32 degrees (which is warmer, but not by much), by day three it tastes off, a at day 5 it was foul- it didn't smell bad, but it sure tasted off. And I used alcohol to disinfect the srage tainer. I would not use tank ammonia as a guide for food spoilage- any cycled tank will quickly process any extra ammonia, and even if it didn't, if you are dosing 40ml into a 200 gallon system, you would need to be dosing food with ammonia concentrations .1-.01 that of household ammonia in order to detect the addition, which you will never have. I will note that you are using salt water for the thaw water, which may be acting as a slight preservative, but I would at least check it. Every batch of mysis (or other food) will be different on it's spoilage time, as each batch starts out with a different amount of bacteria in the product, but maybe reduce storage time by a day and sanitize the container between refills as a precaution