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If it’s a newly set up tank, then yes, you’ll lose most of the beneficial bacteria. You can offset that be adding bacteria from commercial formulations like fritzyme or Dr Tim’s .Hmm.. if I remove sand am I gonna lose all the beneficial bacteria?
Not going to go there. Yes it can - if you test levels - you can be assured that the levels are adequate. There is no way to know for sure - IMHO - whether the sand in xxxx tank is going to absorb copper. The key is keeping the levelpretty sure humblefish said the absorption by sand is minimal. if you have a sand burrowing creature then you can keep it in a small tupperware. but if you dont then i'd get rid of it anyway
I would suggest you call Rubbermaid technical support and ask them. If it does - it's probably minimal. But - why take a chance. Also - a copper test would also help decide whether its safe or notWill copper power absorb into a brute trash can if i mix a batch? or can i just rinse it out afterwards and use for mixing Saltwater in DT?

