I don't think it does. I just cycled a 210 using Dr. Tim's and fishless using his brand of ammonia back in February. It was about a week before I started to see the cycle start and about 30 days before everything was perfect and processing ammonia in less than 24 hours. Directions are below although not formatted the best...sorry. The way I read the instructions when I did mine a couple months back was mix the water, add it to the tank, add some dechlorinator to be safe even though I used RI/RO water, shake the bottle of Dr. Tim's, add it, let it cycle around for about 30 minutes, then dose the ammonia based on the gallons of water I had convert drops to ML and add. I measured ammonia 24 hours later and was pretty much spot on reading 2 ppm. Didn't add any more ammonia until I saw the cycle pattern then ammonia and nitrite read 0. Once that happened I re-dosed ammonia using the same amount per original dose and measured again in 24 hours to make sure it read 0. It did, cycle was done.
At that time I was traveling so left lights and skimmer off. Tank was official in April but mostly due to me being away. The product does work but don't expect it done in a day or two. It will vary but will also be quicker than traditional cycle. Best of luck.
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Directions Set-up your aquarium, filter and heater per the manufacturer’s directions. The One & Only Live Nitrifying Bacteria need a place to live so for best results your tank needs to have gravel or crushed coral on the tank bottom**.
After set-up the water may be cloudy and cold so it is preferable to let the system run overnight before adding the One & Only just to make sure everything is ok. If you have a skimmer, UV and/or ozonizier it’s best to set them up and test the connections now before adding the One & Only. If you are going to use a filter sock you can install it now to remove particles from the water.
Before adding One & Only dose the tank with the correct amount of First Defense to remove chlorine and chloramines, which can harm the One & Only Live Bacteria, from the water. Wait 30 minutes after adding First Defense to add the One & Only. Before adding One & Only you need to remove the filter sock and turn-off the UV, skimmer and ozonizier. These will stay out or off for 48 hours after adding One & Only. To add One & Only shake the bottle well for a few seconds then pour the entire bottle into your tank. You can also add the One & Only to your sump or filter. Your aquarium water may become cloudy but do not worry it will clear in a short time.
Add 1 drop (and one drop only*) of DrTim’s ammonium chloride per gallon of aquarium water. This is Day 1 in the chart below. We recommend NOT using household ammonia. Wait 24 hrs and measure ammonia, nitrite and pH. Record on the chart below - this is day 2. On day 3 add another drop of ammonia per gallon of aquarium water. Measure and record water quality for 2 more days. On Day 6 add another drop of ammonium chloride per gallon of aquarium water. Measure water quality on Days 7 & 8 - in most cases at this point ammonia and nitrite will be zero or below 0.5. Congrats! Your tank is cycled - now you can add some fish and enjoy your aquarium! Follow the schedule on the chart below ending with your first biweekly 25% water change. *Precautions - Do not add ammonia until you get a reading of 2 ppm NH3-N. Do not add ammonia everyday. If the pH drops below 7 perform a 25-30% water change taking the water from near the top of the water column. Do not disturb the substrate or remove the filter pad. If the ammonia or nitrite values are over 5 ppm NH3-N skip the next addition of ammonia drops.