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I think you may have a misunderstanding of how syringes work.
It actually makes NO DIFFERENCE what the air gap is. You could put on a three foot tip, and the liquid would NEVER even enter the barrel, and yet the movement of the plunger will exactly draw in and dispense the amount of fluid that is measured by the travel of the plunger in the barrel. It is the difference in position of the plunger (often most easily measured by the movement of the end of the plunger) after movement that is the only thing that matters.
im still confused. if i pull the ml to the 1 ml mark. and the syringe moves down the the .2 line. does that mean i used .8ml or liquid? or did i use .7 because there was .1 of air in there? its so danged confusing.You know, if I stop and think about it... you are right. The Gap would only make a difference if you where using the entire contents of the syringe. The distance that the plunger moves is the amount of fluid used. Makes total sense now lol
You can’t do that, the scale on the syringe is off by the volume of the tip then.I too had trouble with this . what I do is draw reagent into syringe with tip on . then remove tip empty syringe and draw back into syringe 1ml . then put tip back on seems to work good that way I have exactly 1ml in syringe and tip is full at start of test and end of test so only have to read what was actually used from the syringe. hope this helps
ah ok i thought so, just wanted to be certain. thanks. basically pretend the air is also titration. you count movement of syringe, not just the liquid only.@Reef Puncher
You used .8. 1-.2 = .8.
You do not need to account for the air gap, only mark/measure the distance traveled by the stopper on the syringe.
im still confused. if i pull the ml to the 1 ml mark. and the syringe moves down the the .2 line. does that mean i used .8ml or liquid? or did i use .7 because there was .1 of air in there? its so danged confusing.
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