Wait 24-48 hrs before testing. You are in essence testing for ammonia which present. You want to see drops in ammonia as you are performing tests.
What test kits are you using for testing as API notorious for false readings may be throwing your true number off also.
A typical one week activity with ammonia will go as listed below. there is a difference with using ammonia and ammonia chloride.
- dose ammonia to 2 ppm ammonia-nitrogen [NH3-N] using our ammonium chloride (1 drop per gallon [After Nov 2016 when using DrTim’s ammonium chloride use 4 drops per gallon]) [NOTE: do not expect your test kit to exactly read 2 ppm and it is not critical to get exactly 2 ppm. The key is to not add too much ammonia]. If using DrTim’s Aquatics One & Only Live Nitrifying bacteria add it now (turn skimmer, UV and ozone off and remove filter socks for 48 hours)
- Day 2 – Measure ammonia and nitrite
- Day 3 – If ammonia and nitrite are below 1 ppm add more ammonia: four drops of our ammonium chloride per gallon (check the label)
- Days 4 & 5 – Measure ammonia and nitrite
- Day 6 – If ammonia and nitrite are below 1 ppm add 2 ppm ammonia. Four drops of our ammonium chloride per gallon. [NOTE: since you have added the One & Only your ammonia kit will not read 2 ppm and DO NOT continue adding ammonia trying to get to 2 ppm – just add 2 ppm ammonia (4 drops per gallon of our ammonium chloride) and carry-on
- Days 7 & 8 – Measure ammonia and nitrite. On the first measurement day (Day 2, 4, 5, 7 or 8) that BOTH ammonia and nitrite are both below 0.5 ppm (NH3-N or NO2-N) your tank is close to being cycled.