Hidden Threats - Real and Perceived (Pathogenic Bacteria)

Dennis Cartier

Valuable Member
View Badges
Joined
Aug 25, 2016
Messages
1,970
Reaction score
2,409
Location
Brampton, Ontario
What state or country do you live in
Canada
Rating - 0%
0   0   0
For the last several months I have had a slowly progressing issue in a frag tank that houses my frags, colonies and livestock from a tank that was shutdown from moving. This tank has been setup for a couple of years while a much larger DT is being built. While waiting, I have been testing various nutrient export methods and reefing methodologies on the frag tank to help me to decide what 'style' of tank I wanted the new DT to be and how I wanted to operate it.

However, over time, around the last several months, I have noticed a change in the behaviour of the frag tank. It was a slow subtle change, but in hindsight, each disconnected event all started to make sense when examined as a whole.

The first change that was observed was that my SPS frags stopped growing. At the time, this did not set off any alarms as having growing healthy SPS frags was the outlier rather than the expectation at this point. Other LPS like chalices, were still doing fine at this point.

However, I did notice a change in my cyphastrea frags. This was noted and was of concern. I had both very large cyphastrea colonies that had been grown from dime size frags in the tank along with some recent more rare varieties. The issue started in a large colony where large sections of the tissue would bleach and become translucent, then die. Because these were older colonies that had been under my care for years, I suspected nutrient limitation. But what nutrient? Then my cyphastrea started expressing a new symptom. The polyps would bleach and turn bright white and die while the surrounding tissue would slowly recede. The bleaching would occur in lines moving from polyp to polyp.

The cyphastrea issue was affecting colonies of common varieties at this point. Then the same issue started with chalices. The polyps would turn white, bleach and die, followed by the surrounding tissue. This change got my attention. Hmm, what could it be?

It was around this point, that one of the three bubble tip anemone's that I was keeping started to struggle. It was a larger flame tip that I had had for several years. It started to shrink in size, with stubby tentacles and the oral disc started to become translucent. Normally this one has a dark blue colouration to the tissue around its mouth. Now it was clear with white streaks. I started direct feeding of this anemone and it seemed to improve a bit, but not completely.

Around this time, I noted that the glass was staying very clean with little scrapping required. In fact, I started looking for surface algae and realized that there was very little if any present, while nutrients were still very high (No3 ~ 15 ppm, PO4 ~.12). After thinking about the BTA problem, along with the lack of algae even with elevated nutrients, I concluded that I was most likely Iron limited, so I does some Kent's Iron and Molybdenum. The change in the BTA was very dramatic. Within 3 days, the colours returned to being very dark, the tentacles started extending normally and tentacle loss was reversed. My conclusion was that I was very iron limited and dosing it really helped. For the anemones, it was obviously the iron, but was that also the explanation for the cyphastrea and chalice issue?

Around this time, I added an external fuge with an H380. I had been resorting to using LaCl to help with PO4 export, but decided that using macro algae to export both nitrate and phosphate at the same time, might be a better way to go. With my challenges with Iron, I was sure to keep on top of iron dosing, adding about 10 drops a day. The iron really helped my fugue with accelerated growth, and in no time the NO3 was slowly deceasing along with PO4.

At this point I started seeing some all too familiar tissue loss in my monti caps. I had seen this pattern before, it could only mean nudis. My worst fears were confirmed when I found a nudibranch on one of my caps. Great, that is just what I needed to add to my challenges. I had battled nudis in the past, with depressing results, so I started reading up to see if any new treatments were developed since I had last had them. I started dosing Flat Worm Stop and Coral Booster daily. I know what you are thinking, why would I dose FWS for nudis? This was a technique that some people where reporting they had had good results with. Besides if I had nudis, who is not say that I did not have AEFW in there as well? The change in the caps was noticeable, they started growing fast enough to keep ahead of the nudis. I also added some peppermint shrimp and wrasses. So for the moment detente was declared and the nudis were neither winning nor losing.

I took a 3 gallon jar that I had been planning to make into a nano tank and added it inline with my daily water change output. This jar had a small powerhead and heater and my intention was to use it as an easy way to dip coral frags for the nudis. It did work really well for blowing the nudis off the montis. After a few weeks of being in place, I noticed that the treatment jar developed a huge bacterial mat that covered every surface and piece of equipment in the jar. It looked like a horror movie, where the star walks into a deserted house and everything is covered in cobwebs. I thought it odd, but was not alarmed by it. I figured it might have something to do with the lack of water movement in the treatment jar as the powerhead was off when not being used for dipping.

Around this time I had purchased a large piece (4" x 2") of an encrusting monti that I placed in the tank. Within 2 days, the new monti developed a large white bacterial mat that billowed in the current. The mat covered about 2/3 of the monti surface. I dipped the monti, but once back in the tank, the mat would return. Under the portions covered by the mat was bleached white skeleton. At this point, I was not connecting the dots about the mat and the similar mat in the treatment jar.

By this point, my nutrients were really, really low. My acro frags were not growing. They looked fine, but were just existing. I decided to start dosing nitrate to see if I could raise nitrate, even though my fuge was pulling it all out. I started KNO3 on a daily basis. By having it running on a dosing pump, I could get No3 up to 2 ppm. Things started to look up (growth picked up), but then I started having RTN issues. Literally overnight healthy growing acros and montis would strip. I thought it might be the potassium in the KNO3, so I switched to calcium nitrate. The RTN issues continued. Hmm maybe my chemicals were not contaminant free, so I switched to Seachem Flourish Nitrogen. The RTN continued. I finally stopped all nitrate additions, and the RTN seemed to abate.

In the tank there was an Aquaman encrusting monti that had been growing quite well. It had encrusted the plug it was on and started encrusting onto the ceramic frag rack holding it. However
I noticed a small patch of white skeleton. Oh, oh. Could the nudis have found the Aquaman? I was quite fond of the colouration of this frag, so I decided to dip it before the damage could become too severe. While removing it from the frag rack, the portion that had encrusted on the frag rack broke off and remained on the rack. I dipped the frag and returned it to the tank in the exact same position it had been removed from. The next morning, I awoke to see the recently dipped frag covered in a white bacterial mat. Around this point, the alarms were going off in my head. The mat growing on the aquaman was the same may that had been growing in the treatment tank and on the encrusting monti. I figured it could not be a coincidence. The aquaman frag was a goner a this point, but the encrusted tissue that had not been exposed to the treatment jar was still healthy. Maybe I could grow back from that? Ya, no. Within a couple of days, the all too familiar white bacterial mat consumed it.


At this point, I knew I had an issue with the tank, and could very well lose all the corals, but I was not sure what the exact cause was/is. My nitrate was hovering around 0.2 ppm, so I definitely wanted to raise that, but adding nitrate directly seemed to exacerbate the RTN. I decide to lower the photo period of the fuge. This allowed NO3 to rise to 0.5 ppm, but also allowed some GHA to start growing on the back glass, and the RTN issues picked up again. Gah!

I noticed that without nitrate addition, acros were either healthy and stable and/or growing slowly, but montis were experiencing an odd STN that looked like water colour patches of tissue recession. While googling for possible bacterial causes of RTN/STN I happened across this article https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466290/ and the photos in it had an uncanny resemblance to what I was witnessing on my montis. I state to lean towards my problems being bacterial rather than nutrient level.

I have an ORP probe on this tank. The ORP level was always surprisingly low. It hovered around 250 mv. The measured level did not particularly concern me, but I did note that it was lower than the often observed levels of 350-450 mv reported by others. Just incase the low level was due to the probe being shot, I replaced the probe, but the ORP remained the same.

I started to wonder if all the issues I had been slowly seeing over the past year were caused by a pathogenic bacteria that was attacking the corals. I decided to add a UV sterilizer to see if It would help in this regard. I read up on what other people had experienced when adding a UV. I was hoping that I could monitor the UV's effectiveness through changes in ORP. Other people had reported that their ORP had went up about 10-15% after the addition of a UV.

I ordered a 15W UV sterilizer. This frag tank has a water volume of about 150G when the fuge/cryptic fuge are taken into account, so a 15W UV is quite modest. After installing the UV, I awoke the next day to see that the ORP had increased +100 mv to ~ 350 mv overnight. Wow, way more than I expected. That was 2 weeks ago. Since then the ORP has continued to climb. Hovering around 400-415 mv currently.

With the belief that my issues are largely bacterial in nature, I decided to try to 'reset' my bacterial complement using some of the bacterial additives. I had MB7 on hand, but after researching what was available, I settled on Dr. Tim's Eco-Balance. Mainly because it claims to have probiotics against vibrio. I am not sure my bacterial issues are vibrio, but I know that I do not want vibrio in any case.

I am now dosing Eco-Balance once a week, with the skimmer and UV off for 6 hours after the dose. I have reduced Iron additions to one a week, as I would both like to raise nitrate, so slowing the fuge's growth is desired, and I don't want to have excess iron available for bacterial over growth.

This new regime has only been in practice for a couple of weeks. So far some monti caps have reversed their decline, so that is promising. Not all good news though, other montis have continued unabated. I lost my beach bum yesterday to RTN, so there is still along way to go before I think I have this under control.

Dennis
 
You mentioned phosphates once or so....it would have been useful and interesting to have phosphate reading through the whole way. PO4-limitation is what I suspect for the coral symptoms, but there's not enough evidence to really say it with certainty.

There also is not much information about your other filter ration or the rest of your set up and that would also help

If interested post this information up and I'll keep my eyes open. ;)
 
My phosphate typically is pinned to 0.08, as reported by my Hanna ULR. Sometimes it bounces higher, but rarely lower. I expect that I have a lot of bound phosphate in my cryptic fuge (a 20 gallon barrel holding all the old LR from my tank), so that is most likely why it naturally moves towards 0.08. I am OK with anything between 0.05 and 0.1 for PO4, as running out is not good..

As mentioned this is a bit of an experimental tank. So in the past, it did use bio pellets (All-In-One) and sulfur denitrification. I found the bio pellets to be messy, and the sulfur caused mis-matched dosing requirements between Alk and Calcium. However, nitrate was between 5 and 10 ppm under this regime, and the corals were happy, healthy and growing. I discontinued both of these when I setup the macro fuge. I also have 1 reactor stacked with Siporax still hooked to the tank, and there is a large Marinepure block in the sump. The only other thing the tank runs is a small skimmer NWB-110 (I think).

Lighting is moderate, AP700 peaking at 35%, with 4 T5 bulbs in an Aquatic Life Hybrid fixture. Par is 250 at the top of the tank with only AP700 and 350 when the T5's are on (as reported by my SQ520 meter). Par at the bottom of the tank is 100 to 150 depending if the T5's are on (tank is only 16" deep). T5's run for 3 hours a day. I have been working my way up to extending the T5 period slowly.

Dennis
 
The frag tank in question in independent, or part of the rest of the system described above with the 20 gallon barrel? So there's no "display" tank per se right now....that's really the frag tank for now, right?

(Just getting clear....rereading your first post again now.)
 
So I think you're right, but tell me what you think of this reasoning. If you agree, then it may point to an easy solution.

By this point, my nutrients were really, really low. My acro frags were not growing.

So it was really just low nitrates – that's why it didn't get any worse than this.

Here's my thinking right now.....carbon dosing is a "flood that raises all boats". The flood of carbon gives normally-limited bacterial and other microbes to bloom along with the good guys we think about when we carbon dose.

I think with the nitrate limitation (which sounds like was really getting into nitrogen limitation), the carbon dosing AND the switch to macroalgae (which is another form of carbon dosing due to exudates) that something like you're imagining has gotten a leg up.

Check out Global microbialization of coral reefs as that explains it in detail.

Copeotrophic microbes are the potentially-pathogenic suspects that we risk giving a boost when we mess with the basic biology of the tank.

From the "W":
https://en.wikipedia.org/wiki/Copiotroph
A copiotroph is an organism found in environments rich in nutrients, particularly carbon. They are the opposite to oligotrophs, which survive in much lower carbon concentrations. Copiotrophic organisms tend to grow in high organic substrate conditions. For example, copiotrophic organisms grow in Sewage lagoons.
 
So I think you're right, but tell me what you think of this reasoning. If you agree, then it may point to an easy solution.



So it was really just low nitrates – that's why it didn't get any worse than this.

Here's my thinking right now.....carbon dosing is a "flood that raises all boats". The flood of carbon gives normally-limited bacterial and other microbes to bloom along with the good guys we think about when we carbon dose.

I think with the nitrate limitation (which sounds like was really getting into nitrogen limitation), the carbon dosing AND the switch to macroalgae (which is another form of carbon dosing due to exudates) that something like you're imagining has gotten a leg up.

Check out Global microbialization of coral reefs as that explains it in detail.

Copeotrophic microbes are the potentially-pathogenic suspects that we risk giving a boost when we mess with the basic biology of the tank.

From the "W":
https://en.wikipedia.org/wiki/Copiotroph

That is quite interesting. I have Forest Rohwer's book (Coral Reefs in The Microbial Seas), but it never occurred to me that it could be the macroalgae that is encouraging the pathogenic bacteria in my situation. If it is this pathway, then that would also explain why dosing nitrate to raise NO3 that the macro keeps stripping would have a bad outcome. If all the mouths waiting for NO3 are the dangerous kind, then you are danged if you do and danged if you don't. I was thinking that my iron dosing was being too generous and was tilting things in favour of the bad microbes (This could still be true). Having too low of nutrients caused by the way too powerful fuge, is a dangerous combination. I definitely am very nutrient limited. Yesterday I noticed some burn tips on some digiata's. The Eco-Balance dosing caused a drop in nutrients on the weekend and slowed growth for a day causing an Alk spike up to 9+. My Alkatronic caught it and turned off dosing, but even a short period of time at 9.1x caused burnt tips. I am slowly reducing my alk targets to be between 8.5 - 7.5.

So to recover from this, I would have to either keep my macro's trimmed small to limit the amount of DOC they can provide, or remove them all together. It seems like I am either struggling to keep nutrients down or struggling to keep nutrients up. Finding a happy balance is a challenge.

The addition of the UV seems to have helped, but I expect that it is only a band-aid, not the cure.

You are correct, this system has no DT hooked to it. There are 2 barrels (20G each) holding macro fuge and cryptic fuge, a 30 G sump and a 80 G frag tank. Eventually when I have my new DT system ready (560 G tank currently sitting empty on its stand), this will go back to a simple frag system with less external barrels, etc.

Dennis
 
If all the mouths waiting for NO3 are the dangerous kind, then you are danged if you do and danged if you don't.

It's not the nitrates, it's the byproducts from what is using them up. Actually, it's centered on what's "mostly" using them up. Who's winning the competition, in other words.

In your case the algae are almost surely contributing - the sugars they produce are KNOWN to be able to produce these effects under the correct circumstances.

But it sounds like they did not instigate it, they are just keeping it going.

TIME FOR THE TANK TO GO ON A CARBON DIET!! :)

The upside is that corals do the same thing and are vehemently trying to win the same competition by releasing their own mix of sugars that tend to cultivate their own system of microbial allies. (Which I'm sure have an equal/opposite effect on algae and copiotrophs when the balance is swinging the corals way.)

I think lowering the amount of bio-filter area could help reduce the demand for N to a normal level...maybe allow the elimination of dosing, or at least reduction of. Can you isolate one or both barrels to be their own system and just remove the macro algae for now? It will make extra headache since it becomes another system to heat/maintain so maybe this can't work...
 
I have Forest Rohwer's book (Coral Reefs in The Microbial Seas)

Gonna have to look that one up - love a good book!

If you search my blog for his last name I've got several articles that he authored or co-authored posted there. Didn't realize it until recently when someone else pointed it out to me. ;)
 
It's not the nitrates, it's the byproducts from what is using them up. Actually, it's centered on what's "mostly" using them up. Who's winning the competition, in other words.

In your case the algae are almost surely contributing - the sugars they produce are KNOWN to be able to produce these effects under the correct circumstances.

But it sounds like they did not instigate it, they are just keeping it going.

TIME FOR THE TANK TO GO ON A CARBON DIET!! :)

The upside is that corals do the same thing and are vehemently trying to win the same competition by releasing their own mix of sugars that tend to cultivate their own system of microbial allies. (Which I'm sure have an equal/opposite effect on algae and copiotrophs when the balance is swinging the corals way.)

I think lowering the amount of bio-filter area could help reduce the demand for N to a normal level...maybe allow the elimination of dosing, or at least reduction of. Can you isolate one or both barrels to be their own system and just remove the macro algae for now? It will make extra headache since it becomes another system to heat/maintain so maybe this can't work...

Ok, as a first step, I will remove the Marinepure block and the Siproax reactor. They were left overs from the previous nutrient control regime (back when I had excess NO3). Separating the barrels would be problematic as they need heating and a 'sump' for the fuge pump. It may come to that anyway, hopefully the continued dosing of Eco-Balance can start to move the microbes back into healthy diversity.

Do you think if dosing nitrate along with Eco-Balance (with the UV off) be enough to offset the potential for the pathogenic microbes to utilize it? As the Eco-Balance tends to hit nutrients hard, I would like to provide it a cushion of nutrients for it to use.

Dennis
 
Without knowing what's in it, I'd say to ease off of it or even stop.

It shouldn't be needed if things aren't already too bad off. And if things are too bad already, then I'm pretty sure it won't have the intended effect.

I think I'd keep running the UV for now and just do the pro-nutrient changes you listed so far and see how it goes for at least a few days or even a couple weeks.

If things show no improvement (including nutrients coming into balance) then look for more pro-nutrient changes to make....barrel mods, or something.
 
I think I will also make an effort to keep GAC online and swapped regularly to help with the exudates from the algae fuge. I typically don't run GAC very often, but it should help with the DOC that the microbes are being driven with, or at least that is the theory.

Dennis
 
Ok, as a first step, I will remove the Marinepure block and the Siproax reactor. They were left overs from the previous nutrient control regime (back when I had excess NO3). Separating the barrels would be problematic as they need heating and a 'sump' for the fuge pump. It may come to that anyway, hopefully the continued dosing of Eco-Balance can start to move the microbes back into healthy diversity.

Do you think if dosing nitrate along with Eco-Balance (with the UV off) be enough to offset the potential for the pathogenic microbes to utilize it? As the Eco-Balance tends to hit nutrients hard, I would like to provide it a cushion of nutrients for it to use.

Dennis

I recently removed my own siporax (25 liters) for the same reason (new fuge). What I found was that both nitrate and PO4 went up significantly soon after, which makes sense when you think about it. The media provided a massive amount of surface area for bacteria and the bacteria consumed both nitrate and PO4. I ended up adding the media back and both levels came back down on their own. Nitrate is easier to add than PO4 is to reduce so I prefer to add a little nitrate weekly and it seems to work. If you want to reduce the surface area in your tank, I would just pull out 25% of it at a time and do that gradually over the course of a month of so. If you have lots of live rock it may not matter as much in your case.
 
For the last several months I have had a slowly progressing issue in a frag tank that houses my frags, colonies and livestock from a tank that was shutdown from moving. This tank has been setup for a couple of years while a much larger DT is being built. While waiting, I have been testing various nutrient export methods and reefing methodologies on the frag tank to help me to decide what 'style' of tank I wanted the new DT to be and how I wanted to operate it.

However, over time, around the last several months, I have noticed a change in the behaviour of the frag tank. It was a slow subtle change, but in hindsight, each disconnected event all started to make sense when examined as a whole.

The first change that was observed was that my SPS frags stopped growing. At the time, this did not set off any alarms as having growing healthy SPS frags was the outlier rather than the expectation at this point. Other LPS like chalices, were still doing fine at this point.

However, I did notice a change in my cyphastrea frags. This was noted and was of concern. I had both very large cyphastrea colonies that had been grown from dime size frags in the tank along with some recent more rare varieties. The issue started in a large colony where large sections of the tissue would bleach and become translucent, then die. Because these were older colonies that had been under my care for years, I suspected nutrient limitation. But what nutrient? Then my cyphastrea started expressing a new symptom. The polyps would bleach and turn bright white and die while the surrounding tissue would slowly recede. The bleaching would occur in lines moving from polyp to polyp.

The cyphastrea issue was affecting colonies of common varieties at this point. Then the same issue started with chalices. The polyps would turn white, bleach and die, followed by the surrounding tissue. This change got my attention. Hmm, what could it be?

It was around this point, that one of the three bubble tip anemone's that I was keeping started to struggle. It was a larger flame tip that I had had for several years. It started to shrink in size, with stubby tentacles and the oral disc started to become translucent. Normally this one has a dark blue colouration to the tissue around its mouth. Now it was clear with white streaks. I started direct feeding of this anemone and it seemed to improve a bit, but not completely.

Around this time, I noted that the glass was staying very clean with little scrapping required. In fact, I started looking for surface algae and realized that there was very little if any present, while nutrients were still very high (No3 ~ 15 ppm, PO4 ~.12). After thinking about the BTA problem, along with the lack of algae even with elevated nutrients, I concluded that I was most likely Iron limited, so I does some Kent's Iron and Molybdenum. The change in the BTA was very dramatic. Within 3 days, the colours returned to being very dark, the tentacles started extending normally and tentacle loss was reversed. My conclusion was that I was very iron limited and dosing it really helped. For the anemones, it was obviously the iron, but was that also the explanation for the cyphastrea and chalice issue?

Around this time, I added an external fuge with an H380. I had been resorting to using LaCl to help with PO4 export, but decided that using macro algae to export both nitrate and phosphate at the same time, might be a better way to go. With my challenges with Iron, I was sure to keep on top of iron dosing, adding about 10 drops a day. The iron really helped my fugue with accelerated growth, and in no time the NO3 was slowly deceasing along with PO4.

At this point I started seeing some all too familiar tissue loss in my monti caps. I had seen this pattern before, it could only mean nudis. My worst fears were confirmed when I found a nudibranch on one of my caps. Great, that is just what I needed to add to my challenges. I had battled nudis in the past, with depressing results, so I started reading up to see if any new treatments were developed since I had last had them. I started dosing Flat Worm Stop and Coral Booster daily. I know what you are thinking, why would I dose FWS for nudis? This was a technique that some people where reporting they had had good results with. Besides if I had nudis, who is not say that I did not have AEFW in there as well? The change in the caps was noticeable, they started growing fast enough to keep ahead of the nudis. I also added some peppermint shrimp and wrasses. So for the moment detente was declared and the nudis were neither winning nor losing.

I took a 3 gallon jar that I had been planning to make into a nano tank and added it inline with my daily water change output. This jar had a small powerhead and heater and my intention was to use it as an easy way to dip coral frags for the nudis. It did work really well for blowing the nudis off the montis. After a few weeks of being in place, I noticed that the treatment jar developed a huge bacterial mat that covered every surface and piece of equipment in the jar. It looked like a horror movie, where the star walks into a deserted house and everything is covered in cobwebs. I thought it odd, but was not alarmed by it. I figured it might have something to do with the lack of water movement in the treatment jar as the powerhead was off when not being used for dipping.

Around this time I had purchased a large piece (4" x 2") of an encrusting monti that I placed in the tank. Within 2 days, the new monti developed a large white bacterial mat that billowed in the current. The mat covered about 2/3 of the monti surface. I dipped the monti, but once back in the tank, the mat would return. Under the portions covered by the mat was bleached white skeleton. At this point, I was not connecting the dots about the mat and the similar mat in the treatment jar.

By this point, my nutrients were really, really low. My acro frags were not growing. They looked fine, but were just existing. I decided to start dosing nitrate to see if I could raise nitrate, even though my fuge was pulling it all out. I started KNO3 on a daily basis. By having it running on a dosing pump, I could get No3 up to 2 ppm. Things started to look up (growth picked up), but then I started having RTN issues. Literally overnight healthy growing acros and montis would strip. I thought it might be the potassium in the KNO3, so I switched to calcium nitrate. The RTN issues continued. Hmm maybe my chemicals were not contaminant free, so I switched to Seachem Flourish Nitrogen. The RTN continued. I finally stopped all nitrate additions, and the RTN seemed to abate.

In the tank there was an Aquaman encrusting monti that had been growing quite well. It had encrusted the plug it was on and started encrusting onto the ceramic frag rack holding it. However
I noticed a small patch of white skeleton. Oh, oh. Could the nudis have found the Aquaman? I was quite fond of the colouration of this frag, so I decided to dip it before the damage could become too severe. While removing it from the frag rack, the portion that had encrusted on the frag rack broke off and remained on the rack. I dipped the frag and returned it to the tank in the exact same position it had been removed from. The next morning, I awoke to see the recently dipped frag covered in a white bacterial mat. Around this point, the alarms were going off in my head. The mat growing on the aquaman was the same may that had been growing in the treatment tank and on the encrusting monti. I figured it could not be a coincidence. The aquaman frag was a goner a this point, but the encrusted tissue that had not been exposed to the treatment jar was still healthy. Maybe I could grow back from that? Ya, no. Within a couple of days, the all too familiar white bacterial mat consumed it.


At this point, I knew I had an issue with the tank, and could very well lose all the corals, but I was not sure what the exact cause was/is. My nitrate was hovering around 0.2 ppm, so I definitely wanted to raise that, but adding nitrate directly seemed to exacerbate the RTN. I decide to lower the photo period of the fuge. This allowed NO3 to rise to 0.5 ppm, but also allowed some GHA to start growing on the back glass, and the RTN issues picked up again. Gah!

I noticed that without nitrate addition, acros were either healthy and stable and/or growing slowly, but montis were experiencing an odd STN that looked like water colour patches of tissue recession. While googling for possible bacterial causes of RTN/STN I happened across this article https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466290/ and the photos in it had an uncanny resemblance to what I was witnessing on my montis. I state to lean towards my problems being bacterial rather than nutrient level.

I have an ORP probe on this tank. The ORP level was always surprisingly low. It hovered around 250 mv. The measured level did not particularly concern me, but I did note that it was lower than the often observed levels of 350-450 mv reported by others. Just incase the low level was due to the probe being shot, I replaced the probe, but the ORP remained the same.

I started to wonder if all the issues I had been slowly seeing over the past year were caused by a pathogenic bacteria that was attacking the corals. I decided to add a UV sterilizer to see if It would help in this regard. I read up on what other people had experienced when adding a UV. I was hoping that I could monitor the UV's effectiveness through changes in ORP. Other people had reported that their ORP had went up about 10-15% after the addition of a UV.

I ordered a 15W UV sterilizer. This frag tank has a water volume of about 150G when the fuge/cryptic fuge are taken into account, so a 15W UV is quite modest. After installing the UV, I awoke the next day to see that the ORP had increased +100 mv to ~ 350 mv overnight. Wow, way more than I expected. That was 2 weeks ago. Since then the ORP has continued to climb. Hovering around 400-415 mv currently.

With the belief that my issues are largely bacterial in nature, I decided to try to 'reset' my bacterial complement using some of the bacterial additives. I had MB7 on hand, but after researching what was available, I settled on Dr. Tim's Eco-Balance. Mainly because it claims to have probiotics against vibrio. I am not sure my bacterial issues are vibrio, but I know that I do not want vibrio in any case.

I am now dosing Eco-Balance once a week, with the skimmer and UV off for 6 hours after the dose. I have reduced Iron additions to one a week, as I would both like to raise nitrate, so slowing the fuge's growth is desired, and I don't want to have excess iron available for bacterial over growth.

This new regime has only been in practice for a couple of weeks. So far some monti caps have reversed their decline, so that is promising. Not all good news though, other montis have continued unabated. I lost my beach bum yesterday to RTN, so there is still along way to go before I think I have this under control.

Dennis

One suggestion might be to get an ICP test done to rule out contaminants (Lead for example) and confirm overall water chemistry (Potassium, etc). This sounds frustrating so good luck to you. Will follow to see your final outcome.
 
Let me get this straight. You didnt test your iron levels. You just decided you were low on iron when there is 100 other elements. Then dosed iron and noticed you have flat worms. So you concluded with running UV and dosing iron ? Is this a troll post?
 
Let me get this straight. You didnt test your iron levels. You just decided you were low on iron when there is 100 other elements. Then dosed iron and noticed you have flat worms. So you concluded with running UV and dosing iron ? Is this a troll post?

Yes, I think your post might be a troll ;)

In all seriousness, I made no mention of having flatworms (as I don't), so I think you might be posting into the wrong thread.

Dennis
 
I think I will also make an effort to keep GAC online and swapped regularly to help with the exudates from the algae fuge. I typically don't run GAC very often, but it should help with the DOC that the microbes are being driven with, or at least that is the theory.

Dennis

Agreed, at least it's not a bad place to leave things right now and see how it goes.

If it's not too much more hassle, I'd probably run 1/4 the recommended amount and change it weekly instead of a full load only monthly....at least until you decide how it's going and what to do next.

Definitely trim back the macro drastically too....or even remove it from the system until this has passed.
 
Ok, here is an update on this topic. I have drastically reduced the amount of Iron that I am dosing, down to ~ 0.5 ml/wk. After making this change, I noticed that the nitrate export slowed down for a period of time. This was not surprising, except that the pace of macro growth did not change. I concluded based on this, that the Fe additions were driving more than the macro.

I have kept GAC online during this process, and the cases of RTN for encrusting montipora's has slowed. I have also been doing weekly doses of Eco-Balance to try and re-balance the microbial residents. I am not yet sure how successful or useful this is, it does not seem to hurt, but does slow growth due to the (temporary) reduction in nitrate that follows the doses.

I am contemplating re-adding some limited daily nitrate additions. This take the form of enough calcium nitrate to raise NO3 by <0.5 ppm. Not a huge bump, but considering my NO3 hovers at 0.2 ppm with no dosing, it will be significant. Hopefully this will be well tolerated, and will assist in buffering NO3 for more frequent Eco-Balance additions.

I have been keeping my macros trimmed back more than usual, and my fuge is about 50% dragon's breath now. I use cheato as the carrier for the other macros, and trim back and discard the cheato.

Currently my acro frags are doing quite well. They are growing slowly, coloured up, and are fairly healthy. I am having trouble making enough room for my acro frags to avoid touching each other. When I get them positioned just so, a snail will come along and spin them back into a position that leads to warfare. Ditto with other LPS frags. Only encrusting/plating montipora frags continue to struggle.

Future updates to come.

Dennis
 
If eco-balance is messing with your nutrients like that, I thunk I would stop using it. .2 ppm and .5 ppm are probably indistinguishable due to accuracy/precision if the test itself....I'd read both as effectively zero.

Have you reported a phosphate test number?
 
If eco-balance is messing with your nutrients like that, I thunk I would stop using it. .2 ppm and .5 ppm are probably indistinguishable due to accuracy/precision if the test itself....I'd read both as effectively zero.

Have you reported a phosphate test number?

I tested my phosphate at 0.15 recently. This is about double what I typically test at. I am pretty sure that it has risen as I have trimmed back the cheato more often.

In other news, the monti recession seems to have stopped. I am even getting regrowth on cyphastrea colonies that were like 90% recessed. I am not sure what triggered these developments. The only changes I made were to cut back on dosing Iron, did a few doses of Eco-Balance and tried to keep my macro trimmed more. I suspect the lowered Iron dosing is the most likely of the three.

Dennis
 
Nitrates being limited as they've been can stall some biological processes that ordinarily place some demand on phosphate. (Causing your po4 to rise/accumulate.)

Could be that there was enough die-off to put sufficient nitrogenous waste (ammonia, nitrate, et al) into the water to halt the degradation.

The only thing I'd tweak going forward is nutrients...and only by adding NO3 or PO4 when needed to keep them "in the positive". Leave iron, carbon dosing and the rest alone.

Sounds like even the macro is probably overkill, so keep it small AT LEAST until the system is has a track record of healing for at least a few weeks.

If there's any relapse where corals appear to decline again, I'd consider removing the macro and/or dosing some nutrients to affirm positive levels.

(Corals usually don't have a lot of problems with low N levels if the levels aren't being forced-low and overall conditions are stable....it's unavailable P that's the REALLY big problem.)
 

IF YOU HAD TO TAKE A REEFING EXAM, WOULD YOU PASS?

  • Yes!

    Votes: 32 45.7%
  • Not yet, but I have one that I want to buy in mind!

    Votes: 9 12.9%
  • No.

    Votes: 26 37.1%
  • Other (please explain).

    Votes: 3 4.3%
Back
Top