I think if you are using this in conjunction with vodka dosing, you need to start out with small doses and experiment for your individual tank. I dose Biodigest along with vodka and I use less than the dosage on the box. They are used to get a diverse bacteria into your tank instead of just through vodka dosing. So, the bacteria added with Microbacter7 + that from vodka dosing could = overdosing and bacterial bloom. I know biodigest calls for 1 amp/15 days for my system volume of 100gallons. I dose 1 amp per month. I am actually going to try Microbacter7 when I run out of biodigest. Just a thought.