Then what would be the point of the test? At least as far as this thread goes. The only thing up in the air (from this thread) for debate is whether the bacteria "appears out of nowhere". I've said numerous times, that it's not showing up out of nowhere. It's showing up in response to the ammonia that enters the water via decaying matter (whether from the rocks or
any other source). Select individuals have decided to "debate" with me about this. I'm not sure if it's just that they can't read or what... However, if they have completely read and comprehended what I stated, then the only way to truly test that, would be to do so in a sterile environment.
Now, in terms of what I assumed
@brandon429 was trying to say with
his test. Yes, you would still need a controlled test to compare your results against. Especially
IF the bacteria can still
magically show up in a sterile environment. You'd want to compare the growth rate in the "normal" tank, against the controlled (sterile) tank.