How to proliferate bacteria
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Randy Holmes-Farley
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Can you describe what you are exactly trying to accomplish?
OP
OP
Hello!
Producing large amount of a specific bacteria in lab hemoculture vials instead of buying it over and over again
Randy Holmes-Farley
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Which species? Do you have a pure source?
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I don't know how pure... But one that is sold in blue jars [emoji6]let's not mention names...
Thanks
Randy Holmes-Farley
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There is nothing wrong with mentioning names and I don't know what is in blue containers, but growing them will depend on what type of bacteria they are. Nitrifiers will require ammonia, and nitrite, if you are thinking about quick cycle bacteria, Those designed to metabolize organics will need the organics you intend, etc., as well as a wide variety of other nutrients that are typically described in bacterial medium recipes.
But I think this may end up not working especially well for bacteria that are benthic (grow on surfaces) rather than those that will grow suspended in the water.
With organic carbon dosing, I often found a lot growing on my GAC, and we have often discussed how these would be good starter cultures for other tanks using the same bacteria.
Do you want to sell these cultures?
But I think this may end up not working especially well for bacteria that are benthic (grow on surfaces) rather than those that will grow suspended in the water.
With organic carbon dosing, I often found a lot growing on my GAC, and we have often discussed how these would be good starter cultures for other tanks using the same bacteria.
Do you want to sell these cultures?
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OP
There's 2 types of lab vials one special for bacterias suspended in the water and another special for surface bacterias... Anyways I can't discuss further as I'm starting the process in a couple of days but a friend of mine successfully did it last week.
I'll not sell any, it's just for personal use.
Randy Holmes-Farley
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I can't imagine why you cannot talk about making bacteria for personal use, but I can't help anymore without knowing more. It is also not helpful to others reading this to not explain what you intend.
I'd suggest you just look up detailed media recipes for bacterial cultures and supplement with any special compounds you expect them to metabolize.
I'd suggest you just look up detailed media recipes for bacterial cultures and supplement with any special compounds you expect them to metabolize.
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OP
Dear Randy, sorry I couldn't explain more because I didn't know the exact way I'm going to follow.
Here's a picture of the vials I'm going to use and the type of Bacteria to produce is ZeoBac, which they don't mention details of it.
I'll be testing in two vials because one is specialized in anaerobic bacteria and the other in aerobic.
These vials have a measurement tool on their bottom
My only concern is if the liquid inside these vials is reefsafe?
What I know that it's a kind of protein food for Bacteria.
Thanks!
Randy Holmes-Farley
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I'm no expert on culturing, but I think to culture aerobic bacteria, you'll need to constantly (or at least frequently) aerate the media, or else it will become anaerobic and you'll grow out the wrong bacteria.
I'm not sure what they put into their media, but I can't imagine it isn't reef safe at the dilutions expected. Just some added nutrients and trace elements, probably.
I'm not sure what they put into their media, but I can't imagine it isn't reef safe at the dilutions expected. Just some added nutrients and trace elements, probably.
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OP
OP
Thanks a lot... As I've been told it's some kind of liquid extracted from cow brains plus trace elements
Going back to my college days of microbiology, unless you use some pretty specific sterility protocols, you are likely going to contaminate those vials and/or have one bacteria dominate each culture medium. But bacteria culture media is most definitely good at growing stuff. You might just come up with penicillin or the next new plague.
Naiad
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Haha even using sterility protocols we always ended up with 1 or 2 that somehow got contaminated. You are right that it is very difficult to maintain multistrain plates long term.
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Well the bacteria that we buy in vials don't get contaminated when using the same droplet that comes with it?
I'm just trying to cut my expenses by regrowing it
Randy Holmes-Farley
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Contamination can come out of the air, like a cough or sneeze in the room sending out a mist of little droplets.
Randy Holmes-Farley
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Here I'm providing somehow a constant temperature and light source by attaching the vials to the fuge
Why are you giving them light?
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Tell u the truth... I don't know [emoji4] I imagine that some bacterias need light to work and reproduce... What do you think?
My lab friend told me that it will not negatively affect.
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Randy Holmes-Farley
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They definitely do not need light. If you light it, you may drive the growth of things that are photosynthetic that you do not want, such as cyanobacteria or algae.
Some bacteria you may want, such as nitrifiers, are inhibited by light.
Photoinhibition of marine nitrifying bacteria. I. Wavelength-dependent response
http://www.int-res.com/articles/meps/141/m141p183.pdf
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After waiting around 72 hours, the bacteria in the anaerobic vial successfully bloomed and filled the liquid.
You can check the orange color on the bottom of the vial which means that the process was successful [emoji4]
You can check the orange color on the bottom of the vial which means that the process was successful [emoji4]
