Brandon,
Sorry for the long post, but here goes:
I read through the first 20 pages of this thread, so I think I have a good idea of what you'll say, but wanted to add my data points here and get your thoughts after starting a thread about my own cycle, and then posting in the "Cycling an Aquarium" thread and then stumbling across this post.
Here are the pertinent details:
60 gallon cube, Trigger 20C sump
CaribSea South Seas Dry Rock, ~60 lbs
CaribSea Ocean Direct Sand, 20 lbs
CaribSea Arag Alive Special Grade Sand, 20 lbs
Maxspect Nano-Tech Bio-Spheres, 2.2 lbs (in sump)
Water temp is 79.0°F and salinity is 1.0261, mixed with NYOS salt.
I dosed Dr. Tim's ammonium chloride following the instructions on the bottle n Monday (6/10) night and tested an hour later and ammonia was off the charts at 8ppm (according to API). After panicking and reading a bunch of online threads, I decided to bite the bullet and add my 4oz bottle of Frytz Turbo Start 900.
The next morning (6/11), ammonia was down to 2 ppm. Over on my own cycle thread, I was given the advice to check for the presence of nitrite or nitrate as evidence the bacteria are processing the ammonia. I was able to get an API Nitrite kit next day through
Amazon and already had a Hanna HR Nitrate checker, so on 6/12 I tested for everything again.
Ammonia may have been somewhere between 1 and 2 ppm. I say "may" because obviously this is just a subjective interpretation of extremely similar hues of green. Regardless, and both nitrite (API) and nitrate (Hanna and API) were 0 ppm.
Thursday (6/13), I got the same results, 1-2 ppm ammonia, 0 ppm nitrite, 0 ppm nitrate.
Based on my understanding of the data points in this thread, ammonia control will happen in 10 days with all bottled bacteria except perhaps Brighwell. And with Frytz Turbo it's closer to two days. So in theory, I should be cycled... but my question becomes where did the nitrite and nitrate go?
I saw a drastic drop in ammonia the first night, 8 ppm to 2 ppm (assuming the 8 ppm wasn't a bad result), I've perhaps witnessed a further drop in ammonia but like I said, the greens all sort of blend together when you're trying to track a smaller change that's likely less than the test kit's margin of error/tolerances. So let's conditionally say I've met one condition of being cycled.
I'm clearly past two days. And if I wait until Saturday to do anything, I would be at 5 days, and if I want until 6/20, I'd be at 10 days. So depending on how cavalier I want to be, I could say I've met another condition (time) or will very soon.
So theoretically, I should be able to go to my LFS, buy a paid of clowns, acclimate them, and come back here 24 hours later and post a picture of happy clownfish (I was cycled) or upside down clownfish (I was not cycled), thought I'd likely intervene if things looked like they were going south. My other option would be to add some more ammonium chloride to create a detectable (visually discernible) increase in ammonia, and then look for a reduction, 24 hours later. Presumably at that point there *should* be some nitrites and/or nitrates. I guess a third option is grab some fish and some more bacteria and sleep soundly at night; however, I understand the whole point of this thread is to prevent folks from superfluous purchases... (which is why I am here and not at the store buying more Turbo Start.... yet).
What gives me pause proceeding with the first option is the non-zero chance I got a dead bottle of bacteria, and not wanting to harm a living creature. It was shipped well (insulated with ice pack), and while it did arrive while I was out of town it stayed in a room temp hallway for only two days and then immediately went into my refrigerated (which all my research points to not being an issue), and it looked and smelled like Frytz and other anecdotal accounts said it would.
I did some additional ammonia testing this morning, along with some controls (didn't bother with Nitrite or Nitrate:
A, Tap Water: ~0 ppm
B, Fresh RODI Water: ~0 ppm
C, Fresh Saltwater 0 - 0.25 ppm
D, Tank Water 1: 1 ppm
E, Tank Water 2: 1 ppm
I seemingly have some evidence of the bacteria being alive: 8 ppm ammonia (if real) to 2 ppm... but also have some contradictory data with the 0 ppm nitrites and nitrates. So what would your advice be?