all good info. ive never seen a true stalled cycle in reefing though Ive read of ten thousand api indicated ones, to me that indicates bacteria are so well adapted its really only taken this long for people to find out our withholdings or additions wont do much to life forms that have been self seeding, transporting and attaining feed just fine for aeons its neat spelled that way. a gigantic portion of cycling threads we dig up are from people testing a mix of tank water at the end of several atypical ammonia additions, and if they get mixed nitrite or something then for sure the cycle is claimed stalled
they're all a mix of test param ballparking and wastewater testing, that's why cycles seem to stall but they really don't after you add hydration. You can either do completely unassisted cycling and wait 5 mos for the tank to take on a self seed or you can speed things up to 2 weeks with precise ammonia oxidation testing and dr tims/similar setups.
we do a big water change and retest ammonia in most cases from a clean water palette, then they all comply.
of actions aquarists actually take that hinder cycling it could be medication but we never really see those events paired with a cycle, I vote you can eyeball the ammonia up or down a fair bit and outcome after 30 days submersion is the same (after the big final water change post cycle)
I just read in one of the articles on cycling that some of the bottle bac companies are packing up to 12 ppm in the container ammonia, as holdover.
the dr tim's website says over 5 will suppress, and then our cycling threads with all the crazy ways people change up technique has never seen a stalled cycle.
I dug deep into google scholar in the matter and from what I can tell there's isn't a known ld50 for them because of the variation of biofilm protections, in some sludgewater plants those scum layers allow nitrification to continue though the effluent is 350 mg l amazingly.
in a cold sterile test tube, isolated and suspended cells non associated would be killed by much less levels but they don't present that way in our tanks.