Isochrysis & Tetraselmis storage in the fridge?

[Levinson]

Can Isochrysis galbana or Tetraselmis be stored in fridge as a backup to be cultured later just in case the culture crashes?
I know this can be done with Nannochloropsis but I don't know if it can also be done with Iso and Tetra.

I recently started culturing Isochrysis and plan on culturing Tetraselmis in the future. Luckily my Iso culture seems to be going well so far.
I'm culturing multiple bottles in case it crashes but it would be even better if I can also store them in the fridge as a backup.

 

[Mr. Mojo Rising]

I keep my tetra in the fridge for weeks before I use it to start a culture, its fine.
Never had iso so can't say

 

[Levinson]

Bump..

 

[taricha]

T-iso, I never had luck restoring from fridge or room temp backup. I had to keep multiple live growing containers in case one crashed. It was annoying. Maybe others had better luck.

 

[Levinson]

T-iso, I never had luck restoring from fridge or room temp backup. I had to keep multiple live growing containers in case one crashed. It was annoying. Maybe others had better luck.

Thanks for sharing. Did you use (dose/feed) fridge stored Iso?
I was thinking if the live Iso can't be stored in a fridge, does that mean fridge stored = dead phyto thus may decay in the water when dosed? Not that it's going to be a big problem.

 

[Pistondog]

Can Isochrysis galbana or Tetraselmis be stored in fridge as a backup to be cultured later just in case the culture crashes?
I know this can be done with Nannochloropsis but I don't know if it can also be done with Iso and Tetra.

I recently started culturing Isochrysis and plan on culturing Tetraselmis in the future. Luckily my Iso culture seems to be going well so far.
I'm culturing multiple bottles in case it crashes but it would be even better if I can also store them in the fridge as a backup.

Tetra and nano for sure, rotate the jars every day or 2 to re suspend whats settled on the bottom

 

[taricha]

Thanks for sharing. Did you use (dose/feed) fridge stored Iso?
I was thinking if the live Iso can't be stored in a fridge, does that mean fridge stored = dead phyto thus may decay in the water when dosed? Not that it's going to be a big problem.

The fridge stored the t-iso fine. Still grew lots of pods.
It just didn't grow well to restart a culture after being refrigerated.

 

[Drowned in Salt]

I'm growing 6 strains of phyto ATM and have re started Tiso several time from a sterile bottle kept in a room at room temperature. Longest I've left it was a week though. Looking to expand on that. Was around 20-25c. As for tetra, it resets no problem from the fridge after a month or more. Even without remembering to agitate. Tetra even traveled well to a friend 14 days and started well. I've only killed tetra during the culture not from storage. Tiso is the only ******* we need to resolve how to keep for a month. Im sure a less mature culture would store longer in low light hours and so on