no, do it all at once like Jon:
I’ve decided to perform a rip clean on my 120 with 100% new water. The tank has been running for 5 years and was battling Dino for several months. I used the bacteria method from elegant corals and worked great I haven’t seen Dino in months now. But I tried several other methods including...
www.reef2reef.com
move your stuff right when its this clean, before the re assembly. move only clean items = safest route. relocate no detritus is safest, rinsing the sand in tap water or just replacing it/still pre rinsed is best, all at once shown above.
The hobby has the notion wrong about removing in sections/allowing bacteria to build up elsewhere, they don't.
the live rock is equally powerful whether the sand is removed instantly, or in sections. opposite of common thought. *the prior notion wasn't a big deal until a few sectioned removers recycled and lost their whole tank/disturbed bad zones which can happen.
the rinsing method simply accounts for those rarities, and is harmless to employ owing to updated surface area rules, removing any surrounding media never transmits a remaining bacteria mass to rocks. and if it did, the filtration would be less efficient and not more efficient because more bac = clogged surface area. filtration works most efficiently with the
least amount of bacterial film it takes to cover all surfaces. as soon as more try to stack on, water shear rips them away unless the tank is stagnant. surfaces manage their bacteria independently from other surfaces.
Jon taking a perfectly normal 120 gallon system not in distress and rip cleaning it 5x in one thread proves this fact immensely. he could not have been meaner to that sandbed if he blasted it to the moon and back. skip cycled, because rocks are enough filters and we didn't tap rinse those.