One more data dump for today...
Since AlgaeFix contains 4.5% of a polyquat, that is well above levels harmful to bacteria, so it's possible we might see some level of bacteria suppression with a high overdose of it. And if vibrant in fact has as much of a similar substance in it, then we might see the same.
The caveat is that they are not generally good sterilizers in hard water (saltwater).
So I went looking for any sort of bacterial suppression effect with Algaefix and Vibrant in high doses in tank water....
I took tank water and added 250mg/L of ground up fish flake + 100mg/L of glucose to grow a bunch of bacteria. I added 2 drops/L of methylene blue. The blue color can be used to indicate bacterial activity, because when the bacteria consume all the O2 in the water, the methylene blue gets reduced and loses its color. (google for more:
Methylene Blue Reduction Test)
After 15.8 hrs, the samples were still all fully blue (had not depleted O2 yet).
But by 17.9 hours you could see that the highest levels (1/10) of AlgaeFix and Vibrant had lower or slower bacterial growth than the others, which were already mostly de-colorized.
This effect was very tiny, however and within a half hour the highest concentrations of Algaefix and Vibrant had nearly caught up to the others.
(left to right: AlgaeFix 1/100, 1/30, 1/10)
(left to right: Vibrant 1/100, 1/30, 1/10)
Takeaways:
1) again, Algaefix and Vibrant are weirdly similar - this time on a rate-of-bacterial growth test.
2) 1/10 dilutions of Algaefix and Vibrant delayed rate of bacterial metabolism in richly fed tank water by a small, but detectable and nearly identical amount. There is nothing in the claimed ingredient list of Vibrant that suggests it should delay bacterial growth.
3) the bacterial suppression effect of even a high amount (1/10th dilution) of the polyquat in Algaefix - and whatever is in Vibrant - is really small in saltwater, less than an hour's worth of bacterial growth.
4) It does't seem impossible to keep some spores etc viable in pure AlgaeFix or Vibrant, but that wasn't tested.
5) Concern about the polyquat in algaefix (and perhaps in vibrant) being a risk to saltwater microbiome seems very small.