Nanno culture question

[John Biddle]

I recently began culturing nanno in multiple 2L bottles. So far so good, but I have a question. One culture turned to an olive color, all others are lime green and then darken toward forest green. Is this a problem? Has this culture somehow become tainted/contaminated? Is it still usable as rotifer food. I won't be splitting it, I'll sanitize the bottle and start over, but wanted to know what it was, and if it's harmful.

 

[LiveWire]

Is it possible that the olive colored culture was getting ready to crash?
Are you using an F2 fertilizer or something else?

 

[John Biddle]

I'm using F2 per instructions. This was a newish split 2nd or 3rd day, I forget. Also, maybe releant, there were some small white flake like things at the bottom. Seen this before on good cultures too so didn't worry, but thought I'd mention it.

Culture uses RO/DI water at 1.020. Containers cleaned thoroughly as all others have been. Single bubbler is a rigid airline without an airstone and a moderate bubble rate causing no foam at top of container.