Need help for understanding f2's recipe.

[arman]

Hi guys.
I have problem with this recipe i have.
It says it has 4 stock solution.
I don't understand that should i dissolve for example 75 gr NaNo3 and 5 gr NaH2Po4 to one liter of fresh water or each in one liter separately!

 

[arman]

Cant any one help me?

 

[aaron23]

Cant any one help me?

Why are you using something you don't understand?

I would assume since it says 4 stock solution that they be made in separate containers/different batch

 

[arman]

Because f2 media does not exist here in my country.
I want to try some and see if it works or not.

 

[bblumberg]

Cant any one help me?

OK, I looked at the recipe. It is clear to me that you make the 4 stock solutions listed and then combine them into the final solution at the end. Therefore, you need at least 10 containers: one for each stock solution (1-4), 5 for the metal solutions (one for each) and one for the final mixed solution.

Does that make sense?

Bruce

 

[arman]

Thanks for caring.

You mean i will have how many liters of f2 at last?

 

[bblumberg]

The basic recipe makes 1 L of seawater containing the various solutions. The way I read the recipe is that you make the 4 stock solutions and then combine these 1:1:1:0.5 and make 3.5 mL aliquots (p0rtions) of this mixture. This way, every time you want to make up a liter of medium, you take one 3.5 mL aliquot of the mixture, add this to ~950 mL of seawater then bring the final volume to 1L.

 

[arman]

Thank you very much.You got me out of this misunderstanding.

 

[bblumberg]

You are welcome. Good luck with your algae cultures.

Bruce