https://www.reef2reef.com/threads/the-official-sand-rinse-thread-aka-one-against-many.230281/
what to do with new live sand
my interest was the bacteria question regarding liquid storage
there for sure is a finite date that bottle bac, or bagged bac, or even cross contaminated bac will live in aqueous soln without even extra nutrient support. toss in just a bit of extra nutrients, that time frame ranges. Water vessels will transport and sustain bac that aren't even suited for our tanks, bac loves water mostly.
entire industries exist just to keep water solutions free of bac, not to keep them alive within it, that's the easy part. since we are trading in aerobes, leave a little air gap at the top of that water soln and the bac inside can go a long time, past the due date I agree there would be some loss
-interesting changeup there, however. What is the fuel these guys want....ammonia. if bac dies next to bac, and rots, emits _____ammonia in the container

even the bottles are self sustaining for a while
the adaptive nature of bac has them doing things we'd not assume
here is the best formal study ive seen on bottle bac. extrapolates to bagged and moderately wet pack sand exactly the same:
http://www.advancedaquarist.com/2014/8/review