To onemako - apart for the water discussion, some folks (me) use bio balls and or a porous sponge filter from a cycled tank - yours presumably - nested in there fro a reasonable period, to add to the QT to speed up the cycle - that and water changes to keep the ammonium from biting you. Of course those balls/sponges should not go back into the DT without serious cleaning.
-edit, do not use rocks from the 110 for media support, as they will bind up the Cu you are using for treatment
the water will have bacteria in it, but the important cycling bacteria (de-ammniphying and de-nitrifing) are on the rocks and substrate - welcome and lkeep the questions coming !!