True, sand's ability to house thousands of square feet for bacteria to live on is a benefit. I feel that it's ability to store detritus out weighs it's bacteria housing abilities. So many reefers treat their sand beds like a toxic waste zone... "you can move your tank but DON'T disturb the sand bed, or else...", "every time your stir your sand you will have a mini cycle", "my sand bed is what keeps my nitrates in check". The list goes on. To me, sand is a toxic waste zone and has no place in my tank. A DSB can produce denitrification, but at what risk? The bottom layer of the DSB is the denitrification zone. What happens if the upper layers are disturbed?