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Havent seen that one but from what I have read it needs to be much higher that 25ppm to effect anything, though here they might have used another measure for toxicity other than some damage (possibly just deaths), not too sure25ppm nitrite is known to cause damage in one study on clownfish.
I wouldnt ever look at ammonia or nitrite past when the tank is cycled. Based on the fact that api sells all three together I would guess that is the test kit you have as well, so I would recommend buying a better nitrate test kit as api is trash.
Havent seen that one but from what I have read it needs to be much higher that 25ppm to effect anything, though here they might have used another measure for toxicity other than some damage (possibly just deaths), not too sure
For the moment I’m using salifert test kit.I wouldnt ever look at ammonia or nitrite past when the tank is cycled. Based on the fact that api sells all three together I would guess that is the test kit you have as well, so I would recommend buying a better nitrate test kit as api is trash.
Havent seen that one but from what I have read it needs to be much higher that 25ppm to effect anything, though here they might have used another measure for toxicity other than some damage (possibly just deaths), not too sure
www.reef2reef.com
The 25ppm nitrite damage is from an academic paper on scholar that should be pretty easier to look up if you want. If you search nitrite effect on clownfish google scholar it should be one of the first ones.
While I cannot say with confidence that 25 ppm nitrite is fine in a reef tank, I would make just a few comments on that paper.
25 ppm nitrite is not ever maintained in any operating reef tank. During cycling? Maybe, but it might take effort to do so.
They tested at low salinity. 27 ppt (sg = 1.020). It is well known that it is the salinity that reduces toxicity of nitrite in fish. Perhaps the aquaculture folks they were aiming at use low salinity to save money, but the results may not reflect toxicity in a 35 ppt reef.
While they did see some gill effects at 26 ppm nitrite, it took 100-200 ppm to kill half of the fish over 1-4 days.
I would have been more impressed with the nitrite result reliability if they had tested for ammonia during that experiment. With all that nitrite around in an uncycled vessel, who knows what happens to 25 nitrite in a few days? Maybe some is converted back to ammonia. The symptoms they saw were the same.
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Ammonia and nitrite toxicity to false clownfish Amphiprion ocellaris - Aquaculture International
False clownfish, Amphiprion ocellaris, is one of the most commercialized fish species in the world, highly produced to supply the aquarium market. The high stocking densities used to maximize fish production can increase ammonia and nitrite to toxic levels. In this study, A. ocellaris juveniles...link.springer.com

