Phytoplankton under microscope

[lv_reefer]

My culture can never achieve dark green. After 5 days, it always turns yellowish.
I am using Guillian's F/2 formula, 6500k LED light.
I have taken videos of it under a microscope. Does it look healthy to you experts?

Thank you

2000x

4000x

 

[taricha]

nannochloropsis doesn't move like that.
Looks like tetraselmis. Nice!

 

[Dan_P]

nannochloropsis doesn't move like that.
Looks like tetraselmis. Nice!

What about the color? OK, or not enough nitrogen or too much light?

 

[taricha]

What about the color? OK, or not enough nitrogen or too much light?

I haven't grown tetraselmis specifically, but I don't like that color. The light doesn't look obviously too much, so I'd look closer at the nutrients as you say, if it were mine.

 

[salty joe]

I've cultured tetra and it was deep green like nanno. And I knew tetra was motile but I had no idea they cruise around like that!
You can get a secci stick to measure the density of your culture.
I agree, the color looks way off.

IDK how many watts your light is but I'm guessing you could use more light. Something that's worked well for me is a 12.5W 2700K light bulb inside a white 5 gallon bucket with four 2L bottles of nanno. The bulb touches the bottles and I leave it on 24/7. Artificial light drops off in a hurry so having the bottles up against the light bulb means a lot. I run two buckets like that and harvest 1 1/2 liter daily on an 8 day schedule.

 

[lv_reefer]

the dosing instruction my F/2 is 4ml per gallon. I assume it means add F/2 on day 1 and left it run its course for the entire 10-day period until dark green. Or does it mean to add daily 4ml per gallon?
Anyone of you also add nitrate and phosphate in addition to F/2?
thank you

 

[salty joe]

Yes, dose one time and let the phyto grow.

I don't dose anything else.

I've had cultures that got contaminated stay yellowish instead of turning deep green. Washing my hands and using a spray bottle with isopropyl alcohol to disinfect my funnel, surfaces, etc., helped.

 

[JGT]

Not enough F2. Put it all in at once.Lighting is fine. I have a 40w incandescent bulb on mine and no issues.

 

[martinsp]

My culture can never achieve dark green. After 5 days, it always turns yellowish.
I am using Guillian's F/2 formula, 6500k LED light.
I have taken videos of it under a microscope. Does it look healthy to you experts?

Thank you

2000x

4000x

My culture can never achieve dark green. After 5 days, it always turns yellowish.
I am using Guillian's F/2 formula, 6500k LED light.
I have taken videos of it under a microscope. Does it look healthy to you experts?

Thank you

2000x

4000x

Just sent you a private message.
Call me when you get it.
Thanks!