I don't know that quarantine process entails, but did you give a formalin dip to the fish when they first arrived? If so what was the dose? Were these fish shipped to you or did you pick them up at a local store? Do you know the salinity difference between the water they arrived in and your tank?
High dose formalin dips on newly purchased fish is a really, really bad idea. Formalin is damaging to a fish's skin and gills, plus it removes oxygen from the water - things that you do not want to expose newly arrived fish to!
I've heard people using up to 250 ppm for an hour, and that is too high and too long to be safe for even conditioned fish in tropical temperatures. The highest dose you should use at 80 degrees F. is 167 ppm. I use 150 most of the time. The 250 dose was designed for fisheries biologist for cold water fish, and it got misapplied to tropical fish.
Dosed correctly, methylene blue isn't toxic to fish, but it can kill beneficial bacteria in the biofilter. Used as a dip, its benefit is further limited. Personally, I stopped using that decades ago.
Jay
p.s. - my quarantine protocol is here:
2024 Quarantine Procedures Jay Hemdal David Scarborough Protozoans (Cryptocaryon/ich, Amyloodinium/velvet) and Metazoan trematodes/flukes are by far the most common parasites found on newly acquired fish. A carefully managed, proactive quarantine process can effectively eliminate these...
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