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I could be wrong, but my thinking is that the system is limited by the diffusion of water down into the bed and the more surface area (top of the sand bed) the greater the amount of water that can diffuse down into it. Once you get to a certain depth, all of the NO3 is going to be consumed and it can do no more. You do need a certain depth but additional depth beyond that is of questionable value. I would much rather have more area for diffusion with a 3" bed than a small deep bed.

For denitrification, the sand could fill up with that inert grey sludge that gets into sumps and stuff - I do advocate cleaning it out of sand beds every 4 years, or so, and I supposed that rinsing a bucket every few years can do the same thing.

