I would suggest treating the next one from day one, no matter how good it looks. I think you said you dosed 250 mg/gallon, but maybe that was a typo? It should be 250 mg per 5 gallons. That much cipro would likely kill the anemone as antibiotics do seem a bit stressful on the anemone (they will often bleach to some extent). If you haven't already, read the sticky on treating anemones with cipro as it is a proven method. 100% water change every 12-24 hours. As much flow as you can safely get in your hospital tank will be helpful as well. Good luck. I just lost a beautiful one. I'd had it for 5 months, it arrived with 2 mouths and finally started to split a couple of weeks ago. For whatever reason it didn't complete the splitting process, I'm thinking I should have cut it after a few days as it just declined and turned to mush within about a week of starting to split. It had been absolutely happy before this