well maybe thats the closest to overdose ive seen in 1k cycles
these things are just so fun the test and predict.
it really would be helpful to us as a hobby to see if you sterilized that bacteria, they're really tough. I'd let it stew till saturday then do that full water change calibration / easy for 15 gallons, and by Sunday you'll have some data to help us not have to guess next round.
I kicked up a thread yesterday in the general forum called "things not tested regarding marine filtration bacteria" and your issue is another aspect.
We've tested out to 8 ppm, but not beyond. everyone is guessing at this point fwd until we get some data