One tip on troubleshooting a possible refractometer error. Most "look through" refractometers are ATC models - automatic temperature compensation. However, the ATC mechanism is simply a bi-metal strip that moves the scale inside the refractometer to account for the sensitivity of refractive index to temperature.
For troubleshooting purposes, I'd suggest taking a small amount of your tank water, a small amount of your freshly mixed seawater, the calibration fluid and the refractometer and putting them all in the same place on your kitchen counter. Wait 30 minutes to allow all of the solutions and the refractometer to come to the same temperature. Then calibrate your refractometer with the calibration solution, and measure the tank water/freshly mixed seawater. If they're different than your expectations, it may be that the ATC in your refractometer isn't working properly.
And as, Randy noted, getting another calibration solution my be a worthwhile additional check.