· 72-hours rule: You can do transfers sooner than 72 hours, but you should not go over 72 hours by much; if you inadvertently go over the 72 hours when performing a transfer by more than even an hour, then you must start over again with TTM from Day 1 to be absolutely sure the treatment was effective. See ‘Why/how does it work?’ below for why.
· 12-days rule: The minimum treatment period for TTM is 12 days. This equates to 4 transfers total, every 72 hours. You can do more than 4 transfers within the 12 days as long as (1) the last transfer is performed after 12 days OR longer and (2) you first-and-foremost follow the 72-hours rule above. If you miss a transfer at any point in the process, you must start over at Day 1 to be sure the treatment is effective. Examples of day-combinations that work just fine:
o 4 total changes – 3+3+3+3 = 12
o 5 total changes - 2+3+2+3+2 = 12
o 6 total changes - 2+2+2+2+2+2 = 12
o Daily (24-hour period) changes are discouraged as it may not allow for sufficient time to disinfect the tank and equipment between transfers