I think the general consensus by knowledge hobbyists is that it it significantly better to run the UV from the sump and exit the tank. This way you are assured of getting 3-5 time the turnover rate needed for Protozoa and 10 times or more turnover for algae control. Some are not able to accomplish this and resort to having a loop within the sump. The second best choice would be to draw water from the fist section and then return it to the last section. If this is the direction you need to go, you might have to adjust your flow to the sump from your drain line and Have a faster flow into the tank To compensate.