RO/DI ? or RO ? You are sure this has tested out at 0 ?
When we calibrate our pH probes, we use 7 and 10 because we will be using it in water between 7 and 10. We dont use 2 and 4 to calibrate.
Before I use my refractometer I'll use rodi water to make sure 0ppt is 0ppt, then use 35ppt calibration solution to calibrate it.
You're more than welcome to use just rodi, but then you're not calibrating.
Just because inquiring minds wanting to know.... have you tested your rodi water and fresh salt mix water for Alk?