Acreflavin and hydrogen peroxide for velvet

[nanomania]

Hey, i came across 250mg cp tabs.. here is what it has:

Chloroquine Phosphate IP 250mg
(Equivalent to 155mg chloroquine base)
Colour: Titanium Dioxide IP.

Its round white tablet.. i guess this will be the best way to dose, jus that ill have to crush before using.

 

[Humblefish]

Chloroquine Phosphate IP 250mg
(Equivalent to 155mg chloroquine base)

Just be sure to modify the dosage to reflect the usable chloroquine base. The other 95mg in the tablet is either just a filler and/or the binder.

 

[nanomania]

Just be sure to modify the dosage to reflect the usable chloroquine base. The other 95mg in the tablet is either just a filler and/or the binder.

What about titanium dioxide?

This is what i came across researching about it..

Titanium dioxide nanoparticles enhance mortality of fish exposed to bacterial pathogens.

Highlights
•
First data on the effect of nano-TiO2 pre-exposure on responses to bacterial pathogens.
•
Interplay between nano-TiO2, immune system, histopathology, and bacteria is described.
•
Nano-TiO2 has the potential to affect fish population survival in a disease outbreak.
Abstract
Nano-TiO2 is immunotoxic to fish and reduces the bactericidal function of fish neutrophils. Here, fathead minnows (Pimephales promelas) were exposed to low and high environmentally relevant concentration of nano-TiO2 (2 ng g−1 and 10 μg g−1 body weight, respectively), and were challenged with common fish bacterial pathogens, Aeromonas hydrophila or Edwardsiella ictaluri. Pre-exposure to nano-TiO2 significantly increased fish mortality during bacterial challenge. Nano-TiO2 concentrated in the kidney and spleen. Phagocytosis assay demonstrated that nano-TiO2 has the ability to diminish neutrophil phagocytosis of A. hydrophila. Fish injected with TiO2 nanoparticles displayed significant histopathology when compared to control fish. The interplay between nanoparticle exposure, immune system, histopathology, and infectious disease pathogenesis in any animal model has not been described before. By modulating fish immune responses and interfering with resistance to bacterial pathogens, manufactured nano-TiO2 has the potential to affect fish survival in a disease outbreak.


Further detail:

http://www.sciencedirect.com/science/article/pii/S026974911500192X

 

[nanomania]

Finally i got a manufacturer who sells acriflavine in form of liquid, here are the details:

400ml

Acriflavine 0.1% w/v
Purified water q.s.

Can i use this?

 

[Humblefish]

Finally i got a manufacturer who sells acriflavine in form of liquid, here are the details:

400ml

Acriflavine 0.1% w/v
Purified water q.s.

Can i use this?

I do not believe that would be strong enough. The MSDS for Acriflavine-MS says this:

Acriflavine Neutral <6% CAS #: 8048-52-0
Water >94% CAS #: 7732-18-5

Source: http://www.fishvet.com/Acriflavine MS MSDS pdf.pdf

 

[nanomania]

I do not believe that would be strong enough. The MSDS for Acriflavine-MS says this:


Source: http://www.fishvet.com/Acriflavine MS MSDS pdf.pdf

Well can i dose more instead? How do i calculate? Howmany mg wud it be?

 

[nanomania]

Howmuch 0.1% acri to dose per 10g? The solution is very cheap here, so i dont mind dosing, even if its entire 400ml.

 

[Humblefish]

Howmuch 0.1% acri to dose per 10g? The solution is very cheap here, so i dont mind dosing, even if its entire 400ml.

The standard for using Acriflavine is this product: http://www.fishvet.com/Acriflavine-MS.htm

The recommended dosage for that product is 1 teaspoon per 5 gallons (for a 60 min bath), or 1 teaspoon per 10 gallons (QT treatment). Acriflavine-MS contains (about) 6% Acriflavine Neutral and 94% water as per the MSDS. So, a product containing only 0.1% acriflavine is going to be too diluted for saltwater aquarium use. You would need to dose so much of it that I would worry about lowering the SG, especially when doing a bath.

 

[nanomania]

The standard for using Acriflavine is this product: http://www.fishvet.com/Acriflavine-MS.htm

The recommended dosage for that product is 1 teaspoon per 5 gallons (for a 60 min bath), or 1 teaspoon per 10 gallons (QT treatment). Acriflavine-MS contains (about) 6% Acriflavine Neutral and 94% water as per the MSDS. So, a product containing only 0.1% acriflavine is going to be too diluted for saltwater aquarium use. You would need to dose so much of it that I would worry about lowering the SG, especially when doing a bath.

[emoji17]

 

[Humblefish]

[emoji17]

Honestly, your best bet is to just use the acriflavine powder as needed. If you were needing to give a fish a bath, fill a 5 gallon bucket with SW and dose 200mg of the powder. It will dissolve in just a few minutes, so long as you provide ample circulation, and then you are good to go. Can you get digital scales like the ones below in India?

http://www.amazon.com/American-Weigh-GEMINI-20-Portable-MilliGram/dp/B0012TDNAM

http://www.amazon.com/American-Weigh-Scales-Digital-BL-1KG-BLK/dp/B0012N1NAA

 

[nanomania]

Honestly, your best bet is to just use the acriflavine powder as needed. If you were needing to give a fish a bath, fill a 5 gallon bucket with SW and dose 200mg of the powder. It will dissolve in just a few minutes, so long as you provide ample circulation, and then you are good to go. Can you get digital scales like the ones below in India?

http://www.amazon.com/American-Weigh-GEMINI-20-Portable-MilliGram/dp/B0012TDNAM

http://www.amazon.com/American-Weigh-Scales-Digital-BL-1KG-BLK/dp/B0012N1NAA

Miligram Digi scales are costing 350$ here.. chineese are available but not reliable..

Check this,
http://www.versaquatics.com/treatmentandmethodology.htm

This is what i found from the above site

Acriflavin (Trypaflavin)

Use: Skin turbidity or clouding, mouth rot, fin rot, and disinfection of small wounds.

Spectrum of Action: Costia spp., Chilodonella spp., Trichodina spp., Trichodinella spp., flexibacteria, fin and skin turbidity or cloudiness.

Both names refer to the same drub. It dyes intensely. Acriflavin can be put into the completely set up aquarium but severely damages plants. The filter substrate should be cleaned before addition of the drug. After the treatment, filter over activated charcoal to remove the drug.

Stock Solution: 1 g to 1 liter of water.

Dosage A: 1 ml stock solution to each liter of aquarium water to prevent infections.

Dosage B: 3 ml stock solution to each liter of water for four days to help against infections in the early stage.

Dosage C: 5 ml stock solutions to each liter water in a separate container for two to four days against Columnaris spp., Costia spp., Trichodina spp., and Chilodonella spp.

Dosage D: 10 ml stock solution to each liter water in a separate container for 20 days ( against Ichthyophthirius spp.) or for 10 days (against Oodinium spp.). Caution: Many fish do not tolerate this dosag

 

[nanomania]

400ml × 0.1% = 0.4, means 400mg, so for 10 gallon we require full 400ml bottle..

howmuch sg would fluctuate? Also the bath will just be for 90mints, then transfer to cp. Will that affect the fish negatively?

 

[Humblefish]

400ml × 0.1% = 0.4, means 400mg, so for 10 gallon we require full 400ml bottle..

howmuch sg would fluctuate? Also the bath will just be for 90mints, then transfer to cp. Will that affect the fish negatively?

400ml = 1.69 cups. That wouldn't lower the SG too much (if you used a 10 gal to do the bath), but I would then adjust the QT SG to match so the fish doesn't have to go into a higher SG post bath.

IME; You can transfer a fish in and out of CP without issue.

 

[nanomania]

400ml = 1.69 cups. That wouldn't lower the SG too much (if you used a 10 gal to do the bath), but I would then adjust the QT SG to match so the fish doesn't have to go into a higher SG post bath.

IME; You can transfer a fish in and out of CP without issue.

Yup, that would work.. good idea.. howlong does cp take to dissolve as per ur recomended dose?

 

[Humblefish]

Yup, that would work.. good idea.. howlong does cp take to dissolve as per ur recomended dose?

Almost instantly. I just dump the powder directly into the HOB.

 

[nanomania]

Almost instantly. I just dump the powder directly into the HOB.

Thanks alot..

 

[Squanto Joe]

This is a no joke problem for a tank. I have lost 15 of 19 fish in the last week and a half. If you even suspect that you may have velvet, QT and start treatment. My symptoms did not show right away but my Watanabe pair went in 16 hours. My puffer and clowns hung on the longest and the wrasses are still fighting. Good luck all