Yes, their programme is mix of two things. First the probiotics and second the balling. Now the bio s helps in nitrogen cycle but the in case of probio s it helps in reducing phosphate and nitrate by converting them into biomass which will be skimmed off. So, if you just seed your system with bio S your nitrogen cycle will be fine but there will be phosphate and nitrate which needs to be taken care. So less demanding system can survive with bit more phosphate and nitrate but higher end heavy sps system needs very low level of these so using pro bios will reduce it further. If you are just keeping LPS or very few SPS you can just seed with bio S and then use balling to maintain the level.The "probiotic program" that aquaforest has is the only place I see to use np pro and pro bio s, the one with high demand sps. Under their lps and sps program I doesn't have it listed to use it, just the reef salt, components 123 and the additives amino mix, build, energy and t he other one I can't think of.



