Drawing Hanna reagent for alk question

[ScottR]

When drawing up the reagent for Hanna alk test, how do you know it’s 1mL? Do you pull the syringe all the way to the top where it says 0.1? I watched videos online. Kind of stumped.

 

[KrisReef]

Yes.

 

[ScottR]

Any idea why the 1mL is at the bottom and 0.1 is at the top?

 

[KrisReef]

I don’t understand the latest question. The bottom of the syringe starts at zero when it is empty. As you draw the plunger back it fills the attachment. Pull back and stop at 1ml and the attachment will have 1 ml inside that you then add to the vial, shake and read the meter.

All of the liquid (1ml) in the attachment is supposed to be added to the vial.

 

[ScottR]

When the syringe is in the liquid, 1.0 is at the bottom. Then .9 > .1 -

 

[KrisReef]

Ah. Read the bottom of the black plunger!
This has caused me grief at the 1ml mark because I missed the top end of the scale. You are correct, .1 and zero at the top. Draw back to zero to start.

I think these syringes were designed for dispensing small amounts for infants during vaccinations?

 

[ScottR]

Ah. Read the bottom of the black plunger!
This has caused me grief at the 1ml mark because I missed the top end of the scale. You are correct, .1 and zero at the top. Draw back to zero to start.

I think these syringes were designed for dispensing small amounts for infants during vaccinations?

I see. This caused me a lot of head scratching.

 

[KrisReef]

Yup, I got a lot of funky readings at first until my bad eyes noticed the scale ended without a line at zero on the top side of 0.1.

It’s been a great measuring tool since. I really like it!

 

[ScottR]

Yup, I got a lot of funky readings at first until my bad eyes noticed the scale ended without a line at zero on the top side of 0.1.

It’s been a great measuring tool since. I really like it!

I was so happy to get it. Opening that black box was like Christmas. But salifert reads my alk as 9.6 and Hanna 10.2. But I trust Hanna over myself taking readings using titration.

 

[serwobow]

They're just generic syringes. Probably labeled that way because they will often come pre-loaded with one ml of fluid already in them. People using them can then easily tell how much they injected by just reading the number.

But wait a minute, you fill it all the way to where the zero would be, not the 0.1. OK, now you made me just go re-check the instructions. They say: " Remove the cuvette from the meter and unscrew the cap. Using a 1 mL syringe carefully add exactly 1.00 mL of HI772S Reagent to the sample. Replace the cap and gently invert 5 times. Place the cuvette back into the meter."

To get 1 ml, you have to go all the way to the last hash mark on the pipette. If you go to the 0.1, you will only be taking up 0.9 ml.

I hope the video doesnt show them going to the 0.1, because that would be a mistake.

 

[MnFish1]

When the syringe is in the liquid, 1.0 is at the bottom. Then .9 > .1 -

I see. This caused me a lot of head scratching.

I think this is correct - Full is drawing up to the 0.0 line. thats 1 ml. (But it is an unusual syringe)

 

[ScottR]

They're just generic syringes. Probably labeled that way because they will often come pre-loaded with one ml of fluid already in them. People using them can then easily tell how much they injected by just reading the number.

But wait a minute, you fill it all the way to where the zero would be, not the 0.1. OK, now you made me just go re-check the instructions. They say: " Remove the cuvette from the meter and unscrew the cap. Using a 1 mL syringe carefully add exactly 1.00 mL of HI772S Reagent to the sample. Replace the cap and gently invert 5 times. Place the cuvette back into the meter."

To get 1 ml, you have to go all the way to the last hash mark on the pipette. If you go to the 0.1, you will only be taking up 0.9 ml.

I hope the video doesnt show them going to the 0.1, because that would be a mistake.

When I pulled mine up, it stopped at the 0.01 mark exactly. Couldn’t pull it up further.

 

[ScottR]

Actually, when I get home I’ll check again....

 

[MnFish1]

So - the key is. you dont use the black tip to measure where its stopping - you use the solid black part of the plunger. The smaller part will extend down to .1 or so. Could this be part of the issue?

 

[ScottR]

So - the key is. you dont use the black tip to measure where its stopping - you use the solid black part of the plunger. The smaller part will extend down to .1 or so. Could this be part of the issue?

When I get home I’ll check again. I’m assuming I did it correctly last night because my alk reading was within the normal range

 

[KrisReef]

So - the key is. you dont use the black tip to measure where its stopping - you use the solid black part of the plunger. The smaller part will extend down to .1 or so. Could this be part of the issue?

This is the issue exactly. My old eyes in my dimly lit garage missed the zero more than once. It took me a few times of wild alk changes to figure out what was going wrong. The end of the scale gets hidden in the plunger, and stopping at 0.1 looked correct but was only 0.9ml. I almost feel like going through and making a scale for calibrating the meter reading with the 0.9 syringe error just for fun.

The other issue with the syringe is that it is difficult to push the syringe and land the drops into the vial when you start at zero and try to push the plunger in while holding the vial in a towel trying not to get smudges on the cuvette. That rig is really long with the extension on the end of the syringe.

 

[MnFish1]

This is the issue exactly. My old eyes in my dimly lit garage missed the zero more than once. It took me a few times of wild alk changes to figure out what was going wrong. The end of the scale gets hidden in the plunger, and stopping at 0.1 looked correct but was only 0.9ml. I almost feel like going through and making a scale for calibrating the meter reading with the 0.9 syringe error just for fun.

The other issue with the syringe is that it is difficult to push the syringe and land the drops into the vial when you start at zero and try to push the plunger in while holding the vial in a towel trying not to get smudges on the cuvette. That rig is really long with the extension on the end of the syringe.

Just remember its not the small tip that you have to get to zero.

 

[KrisReef]

Just remember its not the small tip that you have to get to zero.

Actually, the "small tip" starts at the bottom of the cylinder (at zero) where the scale reads 1.0. When you draw the plunger back, the small end needs to go to the end of the scale, (not marked zero) to fill 1.0 mls. The syringe is not ideal for this application, imo.

 

[ScottR]

Actually, the "small tip" starts at the bottom of the cylinder (at zero) where the scale reads 1.0. When you draw the plunger back, the small end needs to go to the end of the scale, (not marked zero) to fill 1.0 mls. The syringe is not ideal for this application, imo.

Do you pull yours as far back as it will go?

 

[Fotocha]

hmm you guys made me question if I'm doing it right