Help: First trident recalibration questions

Detroit_aqua

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So I'm recalibrating my trident for the first time. The lines are already primed with solution and waste and all since it's been in operation for over a month. Do I need to do anything special to recalibrate? Can I leave the reagent bottles connected?

Additionally, my reagent A still has 33 tests, but B and C are empty. When I select full reagent change from the tasks, can I leave the bottle of A? Or will that lead to the trident thinking it has a full bottle of A and start pulling in air when it's empty instead of notifying me I need to change it.

Thanks for reading!
 
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