Here’s My Dummest R2R Question So Far :(

You will end up with the same amount in liquid no matter as long as the black part of the plumger is stopped at the 1 you will have 1ml

That's only true if the air remains in the syringe.

A simple test would be to draw a liquid in for both scenarios and dispense into a measured container, that way you can prove it to yourself and learn to be repeatable. If you expel the air, fill the tip and syringe with fluid with the plunger on 1, it will exceed 1ml.
 
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It doesn't matter if the green cap is on or off. When you start with the syringe all the way pressed out and then suck it back to the 1mm mark, you will have more then 1mm. But when you then push it out (aka displacement), not all of it will go out. You will have some left in the tip of the syringe. Only 1mm of solution leaves the syringe.

1569881016991.png

When you fill up the syringe and pull it back to 1mm like the picture above, the red area fills up first and then the blue area. So, you have more then 1mm of solution in the syringe. But when you push out the solution, you are only displacing 1mm of solution (from the 1mm mark to the 0mm mark.) The red area will still have solution in it even though the syringe is pushed all the way.

If it helps to understand the principle, think of pulling the stopper back to the 2mm mark.
1569881392953.png

If you only want 1mm of solution, you would push the stopper from the 2mm mark to the 1mm mark (blue area). You displace 1mm of solution. The rest of the red area remains full of solution.

So, it doesn't matter what tip you have on. The thing that matter is when you push it forward, push it forward 1 mm.

(and don't add anything to the tip after you have filled it up or it will have empty space in it and mess it up.)
 
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It doesn't matter if the green cap is on or off. When you start with the syringe all the way pressed out and then suck it back to the 1mm mark, you will have more then 1mm. But when you then push it out (aka displacement), not all of it will go out. You will have some left in the tip of the syringe. Only 1mm of solution leaves the syringe.

1569881016991.png

When you fill up the syringe and pull it back to 1mm like the picture above, the red area fills up first and then the blue area. So, you have more then 1mm of solution in the syringe. But when you push out the solution, you are only displacing 1mm of solution (from the 1mm mark to the 0mm mark.) The red area will still have solution in it even though the syringe is pushed all the way.

If it helps to understand the principle, think of pulling the stopper back to the 2mm mark.
1569881392953.png

If you only want 1mm of solution, you would push the stopper from the 2mm mark to the 1mm mark (blue area). You displace 1mm of solution. The rest of the red area remains full of solution.
While I agree with you in principle the syringes are not made to dispense a volume from one volume mark to the other. You will always either have to less or to much volume. You are suppose to draw the volume needed and then dispense the whole volume drawn. So as I tried to explain in my earlier post draw more volume then need with tip attached. Invert get rid of bubbles pushing up plunger while the syringe is inverted. Than turn the tip down and slowly push in plunger until you hit the wanted volume mark. Dispense wanted volume. Discard tip or wash with dead volume or suck in additional air to push out volume in the tip.
 
While I agree with you in principle the syringes are not made to dispense a volume from one volume mark to the other. You will always either have to less or to much volume. You are suppose to draw the volume needed and then dispense the whole volume drawn. So as I tried to explain in my earlier post draw more volume then need with tip attached. Invert get rid of bubbles pushing up plunger while the syringe is inverted. Than turn the tip down and slowly push in plunger until you hit the wanted volume mark. Dispense wanted volume. Discard tip or wash with dead volume or suck in additional air to push out volume in the tip.
I'm afraid you're mistaken. That's why vets and doctors can and do put different sized needles on the same syringe. Even though the needles are different sizes and hold different amounts in them, they can still give an accurate measurement by the displacement of the solution.

I do know and agree that my example is more of a hypothiecal example to understand the principle. In reality, when you pull back the plunger, you will have an air pocket right up against the plunger tip and not solution because there was air in the tip to begin with.
1569884489923.png


So the blue area has air, when you put the tip in the solution and pull back the plunger, that air pocket will be against the plunger tip. The bigger the tip (so if you added the green tip), the more air will be against the tip because of the added air volume. But the principle remains true and in practice, it is accurate. You pull it back to the 1 mm mark and then push it forward, even with that air against the plunger tip, you will have displaced 1 mm of solution. The air will just go back to being in the tip (the blue areas. If that makes you uncomfortable to see that air in there, you can invert the syringe and push out the air like you stated, but you'll either have to over fill the solution and then squirt out the air and the excess (which is what doctors and vets typically do), or draw in more solution after the air's out to get it to the 1 mm mark. After you administer the dosage, you will have leftover solution in your syringe instead of air.
 
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I hope my physician will never draw 5ml into the syringe to inject 1ml into me. Yes if you push the plunger down from the 2ml mark to the 1ml mark it is 1ml of volume coming out of the tip/needle. However these plungers not always glide well and you can easily over shoot that the reason why you get the volume right before you dispense the volume
 
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Wow, what a debate!

A number have said it....tip on, tip off....knocking air out or leaving it in....it doesn't matter, you'll get one ml.

However, if it were me, for more accuracy, I'd use a one ml syringe.

As Edith Ann would say.....
Edith Ann.jpg


"And that's the truth! pffffffffft!"
 
Ok one more thing and than I leave it at it - can’t help it but as a wet lab scientist I get passionate about these things.
What ever you choose to do, in the end what counts more is that you choose one method and do it consistently and stick with it. We humans introduce more error into these tests than any other confounding parameters. Happy testing!!
 
Thanks to one and all. It was, naturally, a serious question, and I truly appreciate all of the wisdom on a seemingly “Duh” kind of question. If I had been a Troll, however... what a great show everyone put on! Peace to all in R2R Land :D
 
okay stop I have the answer let me grab my syringe
edit:I cannot find my syringe but essentially measure the top of the syringe and not the bevel(the cone); at least thats how you learn to read syringes in medical science
 
okay stop I have the answer let me grab my syringe
edit:I cannot find my syringe but essentially measure the top of the syringe and not the bevel(the cone); at least thats how you learn to read syringes in medical science
you wont get your correct dosage if you go to the top of the plunger, its bottom of plunger where the plastics connect. just before the dome.
 
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I apologize that as a grown man I can’t read a syringe— but here’s the embarrassing question:

I need 1ml. I assume that in the top photo I have the plunger pulled back correctly— the widest part (first ring) of the plunger is at the 1ml. mark. But what about the fact that this syringe comes with the green attachment? Is it only 1ml. when when the green attachment is OFF? In the top photo, with the green attachment on, am I getting more than 1 ml.? (If so, how do I know where to situate the plunger in order to compensate?)

Help please o_O
No question is a dum question only those with small minds and big egos would see it that way. It’s better to be safe than sorry. Thanks for asking something someone might be too afraid to because they are more worried about what others would think of them. On your way to successful Reefing! :) but yes as many mentioned with the tip or without the tip if you pull up to the bottom end of the syringe you will get 1 ml. The one rule is though if you are going to use the tip you must have the tip on before taking your sample to ensure accuracy. What are you testing here? Let’s say you pull your sample first and the. You place the tip what’s going to happen is when you are pressing down your filling the tip before your actually dispensing the reagent into the test. I hope this makes sense haha
 
No question is a dum question only those with small minds and big egos would see it that way. It’s better to be safe than sorry. Thanks for asking something someone might be too afraid to because they are more worried about what others would think of them. On your way to successful Reefing! :) but yes as many mentioned with the tip or without the tip if you pull up to the bottom end of the syringe you will get 1 ml. The one rule is though if you are going to use the tip you must have the tip on before taking your sample to ensure accuracy. What are you testing here? Let’s say you pull your sample first and the. You place the tip what’s going to happen is when you are pressing down your filling the tip before your actually dispensing the reagent into the test. I hope this makes sense haha

8E94E2C5-0057-4534-8096-FEF04F79FADC.jpeg
 

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